Articles

Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons

Heterokaryosis is an integral part of the parasexual cycle used by predominantly asexual fungi to introduce and maintain genetic variation in populations. Research into fungal heterokaryons began in 1912 and c...

Authors: Noah B. Strom and Kathryn E. Bushley

Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans

The considerable capacity of filamentous fungi for the secretion of proteins is the basis for multi-billion dollar industries producing enzymes and proteins with therapeutic value. The stepwise pathway from t...

Authors: Martin Schalén, Diana Chinyere Anyaogu, Jakob Blæsbjerg Hoof and Mhairi Workman

Identification and characterization of the ergochrome gene cluster in the plant pathogenic fungus Claviceps purpurea

Claviceps purpurea is a phytopathogenic fungus infecting a broad range of grasses including economically important cereal crop plants. The infection cycle ends with the formation of th...

Authors: Lisa Neubauer, Julian Dopstadt, Hans-Ulrich Humpf and Paul Tudzynski

Simple sequence repeat markers that identify Claviceps species and strains

Claviceps purpurea is a pathogen that infects most members of Pooideae, a subfamily of Poaceae, and causes ergot, a floral disease in which the ovary is replaced with a sclerotium. Whe...

Authors: Barbara S. Gilmore, Stephen C. Alderman, Brian J. Knaus, Nahla V. Bassil, Ruth C. Martin, James E. Dombrowski and Jeremiah K. S. Dung

Production of cyathane type secondary metabolites by submerged cultures of Hericium erinaceus and evaluation of their antibacterial activity by direct bioautography

Fungi of the phylum Basidiomycota are well-known to form a broad spectrum of biologically active secondary metabolites, especially low molecular weight compounds such as terpenoids. Hericium erinaceus produces va...

Authors: T. Shen, G. Morlock and H. Zorn

Geographic information system (GIS)-based image analysis for assessing growth of Physarum polycephalum on a solid medium

The conventional method used to assess growth of the plasmodium of the slime mold Physarum polycephalum in solid culture is to measure the extent of plasmodial expansion from the point of inoculation by using a r...

Authors: Hanh T. M. Tran, Steven L. Stephenson and Jason A. Tullis

Unlocking the potential of fungi: the QuantFung project

Authors: Zsófia Büttel, Rafael Díaz, Benedict Dirnberger, Michal Flak, Sietske Grijseels, Min Jin Kwon, Jens Christian Froslev Nielsen, Yvonne Nygård, Pradeep Phule, Carsten Pohl, Sylvain Prigent, Milica Randelovic, Tabea Schütze, Danielle Troppens and Annarita Viggiano

A framework for an organelle-based mathematical modeling of hyphae

Although highly desirable, a mechanistic explanation for the outstanding protein secretion capabilities of fungi such as Aspergilli is missing. As a result, a rational and predictive design of strains as cell fac...

Authors: Rudibert King

Efficient gene editing in Neurospora crassa with CRISPR technology

Efficient gene editing is a critical tool for investigating molecular mechanisms of cellular processes and engineering organisms for numerous purposes ranging from biotechnology to medicine. Recently developed...

Authors: Toru Matsu-ura, Mokryun Baek, Jungin Kwon and Christian Hong

Phytochelatin synthase is required for tolerating metal toxicity in a basidiomycete yeast and is a conserved factor involved in metal homeostasis in fungi

Phytochelatin synthase (PCS) is an enzyme that catalyzes the biosynthesis of phytochelatin from glutathione. Phytochelatins protect cells against the toxic effects of non-essential heavy metals, such as cadmiu...

Authors: Alaina M Shine, Viplendra PS Shakya and Alexander Idnurm

A new vector for efficient gene targeting to the pyrG locus in Aspergillus niger

The possibility for efficient gene targeting for the controlled integration of DNA constructs is an important tool in fungal genetics.

Authors: Mark Arentshorst, Ellen L Lagendijk and Arthur FJ Ram

Penicillium nalgiovense Laxa isolated from Antarctica is a new source of the antifungal metabolite amphotericin B

The need for new antibiotic drugs increases as pathogenic microorganisms continue to develop resistance against current antibiotics. We obtained samples from Antarctica as part of a search for new antimicrobia...

Authors: K Stefan Svahn, Erja Chryssanthou, Björn Olsen, Lars Bohlin and Ulf Göransson

Next-generation genome sequencing can be used to rapidly characterise sequences flanking T-DNA insertions in random insertional mutants of Leptosphaeria maculans

Banks of mutants with random insertions of T-DNA from Agrobacterium tumefaciens are often used in forward genetics approaches to identify phenotypes of interest. Upon identification of mutants of interest, the fl...

Authors: Kylie Chambers, Rohan GT Lowe, Barbara J Howlett, Manuel Zander, Jacqueline Batley, Angela P Van de Wouw and Candace E Elliott

A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis

Pleurotus sapidus secretes a huge enzymatic repertoire including hydrolytic and oxidative enzymes and is an example for higher basidiomycetes being interesting for biotechnology. The complex growth media used fo...

Authors: Marco A Fraatz, Stefanie Naeve, Vanessa Hausherr, Holger Zorn and Lars M Blank

The capacity of Aspergillus niger to sense and respond to cell wall stress requires at least three transcription factors: RlmA, MsnA and CrzA

Cell wall integrity, vesicle transport and protein secretion are key factors contributing to the vitality and productivity of filamentous fungal cell factories such as Aspergillus niger. In order to pioneer ratio...

Authors: Markus RM Fiedler, Annett Lorenz, Benjamin M Nitsche, Cees AMJJ van den Hondel, Arthur FJ Ram and Vera Meyer

RNA-sequencing reveals the complexities of the transcriptional response to lignocellulosic biofuel substrates in Aspergillus niger

Saprobic fungi are the predominant industrial sources of Carbohydrate Active enZymes (CAZymes) used for the saccharification of lignocellulose during the production of second generation biofuels. The productio...

Authors: Steven T Pullan, Paul Daly, Stéphane Delmas, Roger Ibbett, Matthew Kokolski, Almar Neiteler, Jolanda M van Munster, Raymond Wilson, Martin J Blythe, Sanyasi Gaddipati, Gregory A Tucker and David B Archer

Prospecting the biodiversity of the fungal family Ustilaginaceae for the production of value-added chemicals

Ustilaginaceae (belonging to the smut fungi) are commonly known for their plant pathogenicity. Although these microbes lead to yield reduction of cereal production, they can also have an economically positive ...

Authors: Elena Geiser, Vincent Wiebach, Nick Wierckx and Lars M Blank

Exploring fungal biodiversity: organic acid production by 66 strains of filamentous fungi

Filamentous fungi are well known for their ability to degrade lignocellulosic biomass and have a natural ability to convert certain products of biomass degradation, for example glucose, into various organic ac...

Authors: Nadège Liaud, Christian Giniés, David Navarro, Nicolas Fabre, Sylvaine Crapart, Isabelle Herpoël- Gimbert, Anthony Levasseur, Sana Raouche and Jean-Claude Sigoillot

Welcome to Fungal Biology and Biotechnology

No abstract

Authors: Alexander Idnurm and Vera Meyer

Fungal biology in the post-genomic era

In this review I give a personal perspective of how fungal biology has changed since I started my Ph. D. in 1963. At that time we were working in the shadow of the birth of molecular biology as an autonomous a...

Authors: Claudio Scazzocchio

Identification of the UDP-glucose-4-epimerase required for galactofuranose biosynthesis and galactose metabolism in A. niger

Galactofuranose (Galf)-containing glycoconjugates are important to secure the integrity of the cell wall of filamentous fungi. Mutations that prevent the biosynthesis of Galf-containing molecules compromise cell ...

Authors: Joohae Park, Boris Tefsen, Mark Arentshorst, Ellen Lagendijk, Cees AMJJ van den Hondel, Irma van Die and Arthur FJ Ram

Engineering of Aspergillus niger for the production of secondary metabolites

Filamentous fungi can each produce dozens of secondary metabolites which are attractive as therapeutics, drugs, antimicrobials, flavour compounds and other high-value chemicals. Furthermore, they can be used a...

Authors: Lennart Richter, Franziska Wanka, Simon Boecker, Dirk Storm, Tutku Kurt, Özlem Vural, Roderich Süßmuth and Vera Meyer