ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger
Fungal Biology and Biotechnology volume 4, Article number: 13 (2017)
Fungi are treasure chests for yet unexplored natural products. However, exploitation of their real potential remains difficult as a significant proportion of biosynthetic gene clusters appears silent under standard laboratory conditions. Therefore, elucidation of novel products requires gene activation or heterologous expression. For heterologous gene expression, we previously developed an expression platform in Aspergillus niger that is based on the transcriptional regulator TerR and its target promoter PterA.
In this study, we extended this system by regulating expression of terR by the doxycycline inducible Tet-on system. Reporter genes cloned under the control of the target promoter PterA remained silent in the absence of doxycycline, but were strongly expressed when doxycycline was added. Reporter quantification revealed that the coupled system results in about five times higher expression rates compared to gene expression under direct control of the Tet-on system. As production of secondary metabolites generally requires the expression of several biosynthetic genes, the suitability of the self-cleaving viral peptide sequence P2A was tested in this optimised expression system. P2A allowed polycistronic expression of genes required for Asp-melanin formation in combination with the gene coding for the red fluorescent protein tdTomato. Gene expression and Asp-melanin formation was prevented in the absence of doxycycline and strongly induced by addition of doxycycline. Fluorescence studies confirmed the correct subcellular localisation of the respective enzymes.
This tightly regulated but strongly inducible expression system enables high level production of secondary metabolites most likely even those with toxic potential. Furthermore, this system is compatible with polycistronic gene expression and, thus, suitable for the discovery of novel natural products.
Genome mining has revealed that fungal genomes contain a large number of yet unexplored secondary metabolite biosynthetic gene clusters . Due to next generation sequencing approaches the number of available fungal genomes is steadily increasing as can be seen from the growing number of genomes in the 1000 fungal genomes project . Interestingly, even highly related fungal species contain at least a few unique secondary metabolite biosynthetic gene clusters  and it has frequently been observed that more than one metabolite is produced from a single biosynthetic gene cluster . Therefore, the potential of producing metabolites with interesting pharmaceutical characteristics appears nearly unlimited. However, as secondary metabolites are frequently produced in response to distinct biotic or abiotic stress factors , a large number of the respective biosynthetic gene clusters remains silent under laboratory conditions and, thus, their products unexplored. To exploit the full potential of fungal secondary metabolite production different strategies have been applied [6, 7].
One approach that can be directly applied to cultivable fungal species is the addition of epigenetic modifiers  or co-cultivation with other microbes, which may result in the specific induction of biosynthetic gene clusters . However, while this strategy may lead to the production of novel metabolites, a direct correlation between biosynthetic gene cluster and metabolite product remains difficult. Another strategy is the overexpression of a transcriptional regulator controlling a specific biosynthetic gene cluster . Unfortunately, not all secondary metabolite biosynthetic gene clusters contain a transcriptional activator in direct proximity to their biosynthetic genes , which may hamper this approach. In addition, global transcriptional regulators may overrule the activation from a cluster specific transcription factor as shown for the dihydroisoflavipucine biosynthesis in Aspergillus terreus . While this biosynthetic gene cluster contains a specific transcriptional activator that is indispensable for its activation, the activating effect is overruled in the presence of glucose through the carbon catabolite repressor CreA .
The strategy of targeted activation of cluster specific transcription factors additionally requires the ability for genetic modification of the natural producer strain and may not be suitable for many fungal species. Therefore, recent approaches used the generation of fungal artificial chromosomes (FAC) to clone and transfer whole fungal gene clusters into genetically amenable fungal expression platform strains . In a previous study, 56 gene cluster containing FACs with yet uncharacterised biosynthetic genes from Aspergillus wentii, Aspergillus aculeatus and A. terreus were transferred to Aspergillus nidulans, which resulted in the identification of 17 novel metabolites from 15 different FACs . However, not all gene clusters were successfully activated in the recombinant host, which may be due to the lack of transcriptional activators, repressing conditions or the lack of the correct starter metabolites in the heterologous host.
It has also been shown that induction of secondary metabolite biosynthetic gene clusters in a heterologous host can be achieved by regulating the expression of the global regulator of secondary metabolism LaeA in Aspergillus species . In this respect, a transfer of the biosynthetic gene clusters for monacolin K from Monascus pilosus and terrequinone A from A. nidulans resulted in successful product formation after overexpression of leaA in Aspergillus oryzae . However, induction of several biosynthetic gene clusters appears independent from LaeA control and a specific transcriptional activator in direct proximity to the biosynthetic gene cluster may be lacking. Therefore, a different strategy for gene activation was successfully applied to A. nidulans, in which a serial promoter exchange of each individual gene of a biosynthetic gene cluster was performed. This strategy resulted in the identification of the proteasome inhibitor fellutamide B and its resistance conferring gene inpE . Although successful, this strategy required several rounds of metabolite screening, marker regeneration and subsequent transformation and appears prohibitively time consuming for routine applications.
Due to these challenges it remains difficult to recommend an expression system that allows for high throughput screening for all yet uncharacterised secondary metabolite biosynthetic gene clusters. Heterologous gene expression generally aims for high product yields to elucidate the structure of the metabolite and to analyse its biological activity. A prerequisite for this is the high level expression of target genes, which can be achieved by generating multiple copy integrations, selection of strong promoters or a combination of both [17, 18]. Recently, we introduced a heterologous expression system that uses an Aspergillus niger strain as expression platform that contains regulatory elements from A. terreus . These regulatory elements consist of the terrein biosynthetic gene cluster specific transcriptional activator TerR and its target promoter PterA. When expression of terR is controlled by the A. oryzae amylase promoter and a reporter gene is expressed under PterA control the induction level of the amylase promoter gets amplified through this coupled system . In addition, a SM-Xpress vector has been constructed that allows easy generation of expression plasmids by in vitro recombination with the target gene. This expression system had been successfully applied for the identification of lecanoric acid as product from the A. nidulans orsA gene , has enabled the heterologous in vivo reconstruction of the A. terreus Asp-melanin biosynthetic pathway in A. niger  and was recently successfully used for identification of basidioferrin, which is a novel siderophore produced from a non-ribosomal peptide synthetase (NRPS) that is widely distributed among basidiomycetes .
Another challenge in heterologous expression of secondary metabolite biosynthetic gene clusters derives from possible toxicity of resulting metabolites. Therefore, a tight regulation of gene expression is favoured as it allows for the formation of fungal biomass prior to induction of the expression of target genes. In this respect a tuneable Tet-on/Tet-off expression system has been adapted for use in Aspergillus species [21, 22]. The Tet-on system uses a reverse tetracycline-controlled transactivator that enables titratable induction of gene expression by the addition of the tetracycline derivative doxycycline. In the absence of doxycycline gene expression remains at low background levels, but expression gets strongly induced by addition of doxycycline [21, 22].
As most secondary metabolites are produced from biosynthetic gene clusters, production of the final metabolite generally requires the heterologous expression of more than only one single gene. While a strategy of subsequent transformations with isolated genes accompanied by a marker recycling technique works for clusters comprising only a small number of genes, this procedure is extremely time consuming and probably not suitable for larger clusters containing five or more genes. Therefore, another strategy is the use of self-splicing viral peptide sequences such as the 2A peptide that separates proteins from a polycistronic messenger in different viruses such as the porcine teschovirus-1 (P2A). P2A and similar sequences have been successfully used to separate individual proteins in a range of different eukaryotic organisms [23, 24] among them yeasts such as Saccharomyces cerevisiae [25, 26] and Pichia pastoris . A recent study also used a 2A peptide in Trichoderma reesei, in which the gene coding for the cellobiohydrolase Cel7A from Penicillium funiculosum was combined in a single transcript with the eGFP coding gene to ease screening of cellobiohydrolase positive transformants . Importantly, this technique has also been applied for heterologous production of penicillin in the filamentous fungus A. nidulans by genetic engineering of a synthetic Penicillium chrysogenum penicillin biosynthetic gene cluster . Despite low yields, penicillin K was successfully produced by A. nidulans transformants expressing the polycistronic penicillin biosynthetic gene cluster , indicating that this strategy is suitable for use in fungal secondary metabolite biosynthesis. The suitability of P2A was further confirmed in a recent study on enniatin biosynthesis in A. niger, in which two genes required for enniatin biosynthesis and a luciferase were separated by P2A sequences . While a positioning effect in dependence of the gene order in the polycistronic messenger was observed, all strains produced enniatin and displayed light emission from luciferase activity. Positioning effects were also observed in the cellobiohydrolase expression in T. reesei  and murine cells , indicating that despite polycistronic gene expression the amount of individual proteins may vary depending on the gene order in the expression construct.
Here, we aimed to generate an optimised fungal heterologous expression system by combining the three latter aspects of heterologous secondary metabolite production in A. niger: (1) using the expression amplification system of TerR/PterA under (2) fine-tuneable control of the Tet-on system for expression of (3) polycistronic mRNA of the Asp-melanin biosynthetic genes combined with a fluorescent reporter to study correct subcellular localisation of enzymes.
Integration of Tet-on control into the coupled TerR/PterA expression system
We previously developed a heterologous expression system in A. niger that bases on the transcriptional activator TerR from the A. terreus terrein biosynthetic pathway and its terA (PterA) target promoter . In this combination the induction level of PterA directly depends on the transcriptional level of the terR gene . Furthermore, the activity of the promoter controlling terR expression gets amplified at the target promoter PterA as reporter expression in the coupled system was significantly higher than direct expression of reporter genes . In this first version of the expression system, we controlled terR expression by either the glyceraldehyde-3-phosphate dehydrogenase promoter PgpdA or the amylase promoter PamyB. As both of these promoters derive from primary metabolism, their use may interfere with fungal metabolic physiology. In addition, both promoters are difficult to silence and PamyB shows significant background activity even when A. niger is grown on casamino acids in the absence of any sugars. As this background promoter activity may hamper the production of toxic metabolites, we replaced PamyB in the control of terR by the reverse tetracycline-controlled transactivator (Fig. 1a) containing the fraA promoter sequence for improved cassette stability. The fraA gene encodes a putative ribosomal subunit and had been identified from microarray analyses showing a similar expression pattern as the glyceraldehyde-3-phosphate dehydrogenase and is assumed to be constitutively expressed . The Tet-on:terR construct was used for transformation of the A. niger A1144 strain (Fungal Genetics Stock Center, Kansas, USA) and resulting transformants were analysed for full length single copy integration into the genome (Additional file 1). The resulting expression platform strain ATNT16 (ATNT = A1144 Tet-on:t erR) was analysed for its performance in gene expression.
Analysis of β-galactosidase reporter gene expression
To elucidate the performance of the new Tet-on-controlled expression system in A. niger ATNT16, we generated β-galactosidase reporter strains. Two different constructs were made that both contained the lacZ gene from Escherichia coli as reporter. The first construct contained a fusion of PterA with the lacZ gene (PterA:lacZ) for transformation of the ATNT16 strain (Fig. 1a). The second construct contained a fusion of the Tet-on promoter system directly with the lacZ gene (Tet-on:lacZ) for transformation of the parental A. niger strain A1144 (Fig. 1b). This enabled the comparison of doxycycline dependent gene activation in the coupled amplification system of TerR/PterA under control of Tet-on against the direct reporter gene induction by the Tet-on system. After transformation of the respective A. niger strains, transformants with a single copy integration of the respective reporter construct were identified by Southern blot analysis (Additional file 2) and three independent transformants from each construct were selected for downstream investigation of reporter activities. In addition, three reporter strains from the original PamyB:terR expression platform (P2 strain; terR gene under control of the amylase promoter) with single copy integration of the lacZ gene under control of PterA  were included (Fig. 1c). This allowed comparison of expression properties of the new ATNT16 expression platform with that of the previous platform strain P2. All strains were cultivated for 24 h on 100 mM glucose containing minimal media with 20 mM glutamine as nitrogen source and 1% talc to avoid the formation of cell pellets . For the Tet-on-containing strains parallel cultures were supplemented with various amounts of doxycycline in a range between 0 and 30 µg/ml. All strains were cultivated in two biological replicates and β-galactosidase activity was determined from cell-free extracts in technical duplicates (Fig. 1d). The average specific β-galactosidase activity of the P2 reporter strain on this glucose containing medium was about 6 U/mg, which was in agreement with previous determinations under this growth condition . Both, the ATNT16 reporter strains as well as the Tet-on:lacZ strains only revealed very low background activity when cultivated in the absence of doxycycline (< 0.05 U/mg). Addition of doxycycline to the Tet-on:lacZ strains resulted in a titratable induction of reporter activity, reaching a maximum of 3.4 U/mg at 30 µg/ml of doxycycline. The ATNT16 strain with the PterA:lacZ reporter construct showed significant reporter activity of 2.5 U/mg already at 0.75 µg/ml, which further increased to 17.3 U/mg at 30 µg/ml of doxycycline. This latter activity is about five times higher than the maximum activity obtained from the uncoupled system at 30 µg/ml in which Tet-on directly induces the expression of the target gene. Thus, the ATNT16 expression platform with the terR gene under Tet-on control is tightly regulated in the absence of doxycycline and strongly induced by its addition. However, accompanied with high reporter gene expression, biomass formation in the presence of 30 µg/ml doxycycline in the ATNT16 reporter strains was significantly reduced. This may be due to the high reporter protein production during initiation of germination and seems independent from high levels of activated transactivator protein as the ATNT16 strain without lacZ reporter construct showed no growth defects in the presence of 30 µg/ml doxycycline and β-galactosidase background activity in the ATNT16 strain did not increase by the addition of different doxycycline concentrations (not shown).
Production of aspulvinone E in the ATNT16 expression platform
Our reporter gene analyses indicated that the Tet-on-controlled TerR/PterA system is tightly regulated and allows high level gene expression in the presence of doxycycline. To test whether this also transfers to secondary metabolite production, we used the melA gene, which encodes the aspulvinone E synthetase from A. terreus  under control of PterA and transferred this construct into the Tet-on:terR strain ATNT16. After selection for single copy integration (Additional file 3), strains were cultivated for 48 h in glucose minimal medium either in the absence or presence of 15 µg/ml doxycycline. In accordance with a light yellow colour of aspulvinone E, the cultures grown in the presence of doxycycline turned yellow (Fig. 2a) and the main proportion of the coloured substance solved in the ethyl acetate phase during extraction of culture filtrates (Fig. 2b). In contrast, no obvious yellow colouration of the culture or the ethyl acetate phase was observed in the control cultures without doxycycline (Fig. 2a, b). To confirm that aspulvinone E was produced only under inducing conditions samples were analysed by HPLC using reversed phase chromatography on a C18 column. As shown in Fig. 2c the induced culture revealed a strong signal for aspulvinone E and a minor signal of its stereoisomer isoaspulvinone E . By contrast, only extremely weak background signals were detected in the control culture. These results are in agreement with the β-galactosidase reporter studies and confirm that (1) metabolite production is suppressed in the absence of doxycycline and (2) high yields of metabolites can be achieved under inducing conditions even in strains only carrying a single copy integration of the gene of interest.
Model gene cluster expression from polycistronic mRNA using the P2A peptide
In the next step we aimed in the expression of multiple genes in the Tet-on-controlled TerR/PterA system by engineering polycistronic mRNAs. For a proof-of-concept, the Asp-melanin pathway combined with a fluorescent reporter was used . Asp-melanin is the conidial pigment produced by A. terreus and is distinct from the dihydroxynaphtalene melanin found in conidia of other Aspergillus species. This melanin pigment does not derive from a naphtopyrone precursor that is produced by a polyketide synthase rather than an aspulvinone E synthetase, which is a non-ribosomal peptide synthetase-like (NRPS-like) protein . This pigment biosynthesis pathway appeared most suitable as: (1) Asp-melanin is produced from only two proteins, which are the aspulvinone E synthetase MelA and the tyrosinase TyrP; (2) co-expression of individually controlled genes in the A. niger P2 strain resulted in brown mycelium due to the formation of Asp-melanin, which is easy to visualise; (3) Asp-melanin formation requires the correct subcellular localisation of both enzymes as MelA requires the reducing environment of the cytoplasm and TyrP the oxidising environment of Golgi or ER (4) protein localisation and cleavage efficiency can be visualised by using the red fluorescent protein tdTomato as a reporter.
For the separation of individual proteins during ribosomal translation, the 22 amino acid 2A peptide (P2A, GSGATNFSLLKQAGDVEENPGP) sequence from porcine teschovirus-1 was used , whereby codon sequences of individual P2A peptides were varied on DNA level to allow directed in vitro recombination into the SM-Xpress expression vector that contains the terA promoter, the trpC terminator sequence and a resistance gene for selection of transformants . Two different polycistronic constructs consisting of the melA gene, the tyrP gene and the gene coding for tdTomato were generated to test the efficiency of P2A cleavage and protein localisation in the ATNT16 expression platform (Fig. 3a). For the first construct all three genes were separated by a P2A coding sequence (P2A_P2A construct), which was assumed to result in three individual functional proteins under inducing conditions that lead to brown mycelium and a cytoplasmic localisation of tdTomato as this reporter does not contain a subcellular localisation signal. The second construct only contained a single P2A sequence (P2A construct) separating the melA and tyrP genes, whereby the gene coding for tdTomato was fused in frame with the tyrP gene . Here, we expected the formation of brown mycelium under inducing conditions, but a fluorescence localisation in subcellular organelles of ER and Golgi, which would confirm the correct targeting of TyrP. ATNT16 was transformed with the respective constructs and resulting transformants were analysed by Southern blot analysis (Additional file 4) for single copy integration. For analysis of selected transformants split plates were prepared with glucose minimal medium containing 0 or 10 µg/ml of doxycycline. On these plates the control strain ATNT16 as well as strains containing either the P2A or the P2A_P2A construct were spotted and pictures taken after 72 h of incubation. As shown in Fig. 3b, all strains showed similar growth and conidia formation in the top view of plates. However, the bottom view shows that mycelium of strains with the P2A and the P2A_P2A construct turned dark brown. Similarly, liquid cultures inoculated with conidia of the respective transformants were incubated for a total of 24 h in absence (Doxy 0 h) or presence of 15 µg/ml doxycycline. Thereby, the inducer doxycycline was added either directly at the start of cultivation (Doxy 24 h) or after a pre-cultivation for 18 h to allow for conidia germination and hyphae formation prior to induction resulting in an induction time of 6 h (Doxy 6 h). In the absence of doxycycline mycelia of cultures containing either of the two different constructs remained uncoloured, whereas mycelia turned brown under inducing conditions even when induced for only 6 h (Fig. 3c). Therefore, both constructs produce functional proteins that produce Asp-melanin and regulation of gene expression is active on solid and in liquid media.
Expression of full length polycistronic mRNAs
To confirm that all genes from the polycistronic mRNA were expressed with high efficiency only under inducing conditions, we aimed in semiquantitative RT-PCR analyses on the P2A_P2A transcript. Total RNA was isolated from liquid cultures containing the ATNT strain with the P2A_P2A construct. The cultures were grown for 24 h either without doxycycline or were induced for 6 or 24 h. cDNAs were generated with anchored oligo(dT) primers and cDNA levels from the different cultivation conditions were normalised against the A. niger actin gene. Oligonucleotides were deduced that amplify regions of the three individual genes that are contained in the polycistronic transcript (Fig. 3d). As shown in Fig. 3e, in the absence of doxycycline (Doxy 0 h) no amplification was observed on the 5′ and middle region of the polycistronic transcript encoding MelA and TyrP and only a weak band was detected for the gene sequence coding for tdTomato. This is in agreement with the low basal expression observed in lacZ reporter assays and in analysis of aspulvinone E metabolite production. However, strong induction was observed from both induced cultures (Doxy 6 h and Doxy 24 h) with PCR products on all three gene regions from the polycistronic messenger. This indicates that the full-length mRNA is efficiently transcribed only under doxycycline inducing conditions.
Subcellular localisation of proteins
Colouration of the mycelium indicated functional production and separation of MelA and TyrP and transcript analyses showed that all three genes encoded on the single transcript were efficiently transcribed. However, these analyses did not confirm the correct separation of proteins from the second P2A peptide, which should result in a cytoplasmic localisation of tdTomato, nor confirmed the correct subcellular localisation of any of the proteins. Therefore, fluorescence microscopy analyses were performed. While the supplementation of liquid media with talc avoids pellet formation, it hampers microscopic analyses of hyphae due to the attached talc particles. On the other hand, individual hyphae are difficult to visualise once fungal pellets are formed. As our analyses showed that Asp-melanin is formed either on liquid or solid media, strains were grown on glucose minimal media containing agar plates and coverslips coated with doxycycline containing glucose minimal media were placed around the colonies. Once hyphae grew on the edges of the coverslips they were removed, embedded in a DAPI-containing mounting solution and analysed by fluorescence microscopy. As shown in Fig. 3f hyphae of the strain containing the single P2A separator, which means a fusion of the tyrP gene with the gene coding for the red fluorescent protein tdTomato, exclusively showed red fluorescence in subcellular organelles most likely resembling Golgi and ER . This indicates that TyrP is quantitatively transported into the correct subcellular compartment after P2A cleavage from MelA and a functional TyrP-tdTomato fusion protein is produced. In contrast, hyphae of the strain containing the P2A_P2A construct showed very strong cytoplasmic red fluorescence, indicating that both P2A cleavage sites were correctly recognised and full length tdTomato had been produced. Observation of colonies grown in the absence of doxycycline only revealed DAPI staining of nuclei but no red fluorescence signal (not shown).
The aim of this study was the generation of an optimised fine-tuneable expression system in A. niger that produces high expression rates when fully induced and which is suitable to express polycistronic genes for recombinant expression of fungal secondary metabolite biosynthetic gene clusters. Previous studies investigated the suitability of A. niger as expression platform for the production of secondary metabolites such as the non-ribosomal peptide enniatin . Thereby, the use of the Tet-on expression system combined with optimised fermentation conditions resulted in 4.5 g/l enniatin confirming both, the suitability of the Tet-on system to induce secondary metabolite production and the suitability of A. niger as expression platform. Similarly, we have previously shown that a range of different metabolites such as polyketides , non-ribosomal peptides  and products from NRPS-like enzymes  can be successfully produced in A. niger.
The combination of our TerR/PterA-system with the Tet-on-system resulted in an expression system with exceptionally high transcription rates which are still titratable. The coupling of Tet-on with the highly specific transcription factor TerR resulted in an amplification of gene expression by more than 5 times compared to direct gene expression from Tet-on. Therefore, even single copy integrations result in high transcript levels, which makes a selection for multi copy integration strains dispensable  and reduces adverse effects on growth and physiology caused by multiple random genome integration events. However, as our approach did not target a specific gene locus, some positioning effect from independent single copy integration strains was observed, which is resembled in the standard deviations from β-galactosidase activity determinations.
Another advantage of using the coupled Tet-on controlled TerR/PterA expression system compared to direct expression from the Tet-on promoter system is the low concentration of doxycycline required for induction of gene expression. Significant expression rates were already observed at 0.75 μg/ml doxycycline. This activity from the coupled system was similar to that obtained from the direct Tet-on controlled gene expression at 15 µg/ml. Low amounts of doxycycline reduce the risk of co-extraction of the inducer when aiming for purification of secondary metabolites. However, as highest expression rates were observed at 15 to 30 µg doxycycline, these should be used especially when producing toxic metabolites that require significant biomass production prior to high level induction of gene expression. As a proof-of-concept we showed that Asp-melanin is efficiently produced when strains were pre-grown for 18 h and induced by doxycycline for only 6 h.
The tight regulation of the Tet-on controlled TerR/PterA expression system combined with its high induction rate makes it also superior to our original PamyB controlled TerR/PterA expression system, which is constitutively active on sugar containing media . As glucose containing medium is generally used in the regeneration of protoplasts in fungal transformations, the production of toxic natural products may prevent growth of positive transformants. In contrast, regeneration of protoplasts of ATNT16 strain in the absence of doxycycline prevents expression of heterologous genes. In this respect, when we expressed the aspulvinone E synthetase gene melA in the A. niger P2 strain (PamyB control of terR expression) we suffered from the reduced ability of fungal colonies to produce conidia . In this study, ATNT16 melA OE strains grown in the absence of doxycycline were indistinguishable from the parental control strain, unless induced by doxycycline.
Importantly, the P2A polycistronic gene expression was compatible with our high level expression system. Asp-melanin was efficiently produced from both, the single P2A construct that contained the fusion of TyrP with tdTomato as well as the construct in which all three proteins were separated by P2A sequences. While we were not able to quantify the production of the insoluble pigment Asp-melanin, the brown colouration of the mycelium in the single P2A construct appeared less pronounced compared to the P2A_P2A construct (Fig. 3b, c). Positioning effects due to the order of genes in the polycistronic messenger as described previously [28, 30] that may in part be due to a drop-off of the ribosome after the translational skipping event  cannot account for this difference as the position of the tyrP gene was identical in both constructs. Therefore, it is likely that the fusion of TyrP with tdTomato affects activity of the tyrosinase. Nevertheless, this fusion unambiguously showed that the TyrP protein is correctly and quantitatively targeted to the ER and Golgi as (1) TyrP is inactive in the reducing environment of the cytoplasm  and therefore needs to be transported to the oxidative environment of Golgi and ER and (2) no cytoplasmic background fluorescence from tdTomato remaining in the cytoplasm was observed. Therefore, the recognition of the N-terminal subcellular localisation sequence has not been affected by the proline residue added to TyrP from the P2A peptide  when cleaved from the Aspulvinone E synthetase MelA. Whether C-terminal localisation sequences such as the peroxisomal PTS1 tripeptide import sequence SKL or AKL, as found in proteins of fungal siderophore biosynthesis , may be masked by the C-terminal addition of a P2A sequence needs to be tested in future studies.
This study also showed that the second P2A sequence in the P2A_P2A construct is efficiently cleaved as this construct showed extremely bright red fluorescence from the cytoplasm. However, due to the extremely bright fluorescence from this construct, which even leads to a reddish appearance of the edges of colonies on plates, we cannot exclude that some uncleaved protein may still be transported into the ER and Golgi. Nevertheless, due to the high fluorescence intensity from the cytoplasm combined with the high activity of the tyrosinase from the P2A_P2A construct, the majority of P2A peptides has efficiently been cleaved.
The combination of tightly controlled Tet-on induction with the highly specific TerR/PterA expression system resulted in a well-regulated fine-tuneable and very strong gene expression. Therefore, the system appears suitable for high-level production of metabolites, even those with antifungal properties. In addition, the system is compatible with the use of self-cleaving peptides such as P2A. Cleavage sites are efficiently recognised and at least N-terminal secretion signals seem to remain unaffected. Therefore, this system can be used for the discovery of metabolites from yet unexplored fungal secondary metabolite biosynthetic gene clusters.
Media, fungal cultivation and transformation
Conidia suspensions were obtained by growing Aspergillus niger strains in slope cultures containing Aspergillus minimal medium with 50 mM glucose as carbon and 10 mM glutamine as nitrogen source  denoted as GG10 medium. For solid media 2% agar was added. Slopes were incubate for 4 days at 28 °C and overlaid with 6 ml phosphate-buffered saline (PBS) containing 0.01% Tween 20. Conidia were scraped into suspension using sterile cotton swaps. Suspensions were filtered over a 40 µm cell strainer (Greiner BioOne) to remove hyphae and clumps of conidia. After centrifugation the supernatant was discarded, conidia suspended in PBS and conidia concentrations determined using an improved Neubauer chamber. If not indicated otherwise, GG10 liquid cultures were inoculated with 1 × 106 conidia/ml with or without the addition of doxycycline (final concentration 0–30 µg/ml) and incubated at 28 °C on a rotary shaker at 150 rpm. Mycelia and culture supernatants were separated by filtration over Miracloth filter gauze (Merck, Calbiochem). Mycelia were pressed dry between tissue paper and frozen in liquid nitrogen for subsequent analyses. Fungal transformation was performed as described previously  with some minor modifications. Mycelia for protoplast formation were generated by inoculating YEPD medium (20 g peptone, 10 g yeast extract, 5 g glucose per litre) with spores of the A. niger wild-type strain A1144 (Fungal Genetics Stock Center, Kansas, USA) or the expression platform strain ATNT16. After 22 h mycelia were washed and incubated for 60 min in 90 mM citrate–phosphate buffer pH 7.3 containing 10 mM dithiothreitol. Protoplasts were generated by using 1.3 g/20 ml sterile filtered VinoTaste Pro (Novozymes) in osmotic medium with 0.6 M KCl as osmotic stabiliser. After transformation protoplasts were regenerated on solid GG10 media containing 1.2 M sorbitol and either 40 µg/ml phleomycin, 140 µg/ml hygromycin B or 0.1 µg/ml pyrithiamine as selectable marker. Genomic DNA was isolated using the MasterPure Yeast DNA purification kit (Epicenter).
Generation of the ATNT16 expression platform strain
All oligonucleotides used in this study are listed in Additional file 5: Table 1. All PCR reactions were performed in a SpeedCycler2 (Analytic Jena) in 10 µl volumes using either Phusion (fragment cloning, Thermo Scientific) or Phire Hot Start II DNA polymerase (colony PCR, Thermo Scientific) for DNA amplification. PCR fragments and digested plasmids for cloning purposes were gel purified using the GeneJet Gel Extraction Kit (Thermo Scientific). To generate the A. niger ATNT16 strain a plasmid was generated that contained the gene of the transcriptional activator terR under control of the Tet-on reverse transactivator system . The construct was cloned into the HindIII linearized pUC19-ble  vector containing a phleomycin resistance cassette for selection of transformants. The Tet-on system was amplified with oligonucleotides 1 and 2 from plasmid pFW22.1 (kindly provided by V. Meyer, Berlin) and contained overhangs to the HindIII site of pUC19-ble. Subsequently, the terR gene including its own terminator sequence was amplified with oligonucleotides 3 and 4 from genomic DNA of Aspergillus terreus SBUG844 and cloned into the NcoI linearized TetOn_ble_pUC19 vector . The 5′-end contained an overhang to the Tet-on system and at the 3′-end to the HindIII site of pUC19-ble. Linearized plasmid and gel-purified PCR products were mixed and assembled by in vitro recombination using the InFusion HD cloning kit (Takara/Clonetech) resulting in plasmid Tet-on:terR_ble_pUC19. The assembled plasmid was amplified in Escherichia coli DH5α using Mix & Go competent cells (Zymo Research). Positive clones were selected by colony PCR using oligonucleotides 5 and 6. Plasmids were isolated by use of the NucleoSpin Plasmid Miniprep kit (Macherey-Nagel) and correct assembly was confirmed by restriction analyses. The plasmid was used for transformation of A. niger A1144 and phleomycin resistant transformants were checked for single copy integration of the construct by Southern blot analysis using a dig labelled probe amplified with oligonucleotides 7 and 8. Transformant ATNT16 was selected for subsequent studies.
Generation of lacZ reporter and aspulvinone E synthetase gene expressing strains
To generate the lacZ reporter strains in the ATNT16 background, plasmid hph_tdTomato:lacZ:trpC T_pJET1.2  containing the lacZ reporter under control of the terA promoter and a hygromycin B resistance cassette were used for transformation of ATNT16. A fusion of the Tet-on transactivator with the lacZ gene was assembled in the PstI restricted pUC19_ptrA plasmid  containing the pyrithiamine resistance cassette as selectable marker. Tet-on was amplified from plasmid pFW22.1 with oligonucleotide 9 containing an overhang to the PstI site of pUC19_ptrA and oligonucleotide 10. The lacZ gene including a trpC terminator was amplified from plasmid hph_tdTomato:lacZ:trpC T_pJET1.2 with oligonucleotide 11 containing an overhang to the 3′-end of Tet-on and oligonucleotide 12 with overhang to the PstI site of pUC19_ptrA. Linearized plasmid and the two gel-purified PCR fragments were mixed, assembled by in vitro recombination and transferred to E. coli as described above. Positive clones were selected by colony PCR using oligonucleotides 13 and 14. Isolated plasmids were checked by restriction analyses and used for transformation of A1144. Genomic DNA of ATNT16 and A1144 transformants was restricted with SmaI and analysed by Southern blot with a probe against the lacZ gene (oligonucleotides 13 and 14). At least three strains with a single copy integration of the reporter construct were used for expression analyses. For expression of the A. terreus aspulvinone E synthetase gene melA in the ATNT16 background, plasmid his_melA-SM-Xpress  was used as it contains a fusion of the terA promoter with the melA gene. The phleomycin resistance cassette of this plasmid was excised by NotI restriction and replaced by the pyrithiamine resistance cassette (ptrA) for transformation of ATNT16. Transformants were analysed by Southern blot with a probe against the melA gene (oligonucleotides 15 and 16) and strains with single copy integration were selected (Additional file 3).
Generation of model gene cluster expressing strains
Two different polycistronic expression constructs were generated for gene expression in ATNT16. The first construct contained the melA gene and a fusion of the tyrP gene and the gene coding for tdTomato. The melA and tyrP genes were separated by a P2A coding sequence. The melA gene was amplified from genomic DNA of A. terreus SBUG844 with oligonucleotide 17 that contained an overlap to the NcoI restricted SM-Xpress2 vector  and oligonucleotide 18 with an overhang coding for a the P2A sequence. The gene fusion of tyrP with the tdTomato gene was amplified from plasmid tyrP:tdTomato_SM-Xpress2  with oligonucleotide 19 possessing an overhang to the P2A sequence and oligonucleotide 20 with an overlap to the NcoI restricted SM-Xpress2 vector. In the second construct all three genes were separated by P2A sequences. The melA gene was amplified with the same oligonucleotides as for the first construct. The tyrP gene was amplified from genomic DNA of A. terreus SBUG844 with oligonucleotide 19 containing the P2A sequence overhang towards melA and oligonucleotide 21 with a P2A sequence overhang towards the tdTomato gene. Finally, the tdTomato gene was amplified from plasmid tyrP:tdTomato_SM-Xpress2 with oligonucleotide 22 possessing the complementary overhang to the tyrP 3′ P2A sequence and oligonucleotide 20 with a compatible overhang to the SM-Xpress2 vector. Constructs were assembled by in vitro recombination and transferred to E. coli DH5α. Clones were checked by colony PCR using oligonucleotides 23 and 24 to test for correct gene assembly. Plasmid DNA was isolated and used for transformation of ATNT16 with hygromycin B as selectable marker. Transformants were analysed by Southern blot with a probe against the melA gene (oligonucleotides 15 and 16) and strains with single copy integration were analysed further (Additional file 4).
β-Galactosidase reporter assays
To study β-galactosidase reporter activities, fungi were inoculated at 2 × 106 conidia per ml and grown for 24 h in 100 mM glucose containing minimal media with 20 mM glutamine as nitrogen source and 1% talc to avoid the formation of cell pellets. Mycelia were harvested over Miracloth, pressed dry and frozen in liquid nitrogen. Mycelia were ground to a fine powder under liquid nitrogen and suspended in Z buffer (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, 0.7% β-mercaptoethanol). After centrifugation for 5 min at 16,000 × g and 4 °C the cell-free supernatant was removed and used for determination of β-galactosidase activity as previously described  using o-nitrophenyl-β-d-galactopyranoside (ONPG; ε = 3.5 mM−1 cm−1) as substrate. Protein concentrations were determined by using the Bradford Protein Assay (BioRad) with bovine serum albumin as standard. All spectrophotometric assays were carried out using an Evolution 220 UV–VIS spectrophotometer (ThermoFisher Scientific). From each construct three independent strains were grown in biological duplicates and activity determinations were made in technical duplicates.
Analysis of aspulvinone E production
To test production of aspulvinone E in ATNT16 strains carrying a single copy integration of the PterA:melA construct, GG10 medium was inoculated with 1 × 106 conidia/ml and one culture was supplemented with 15 µg/ml of doxycycline, whereas the other was left untreated. Incubation was performed for 48 h at 28 °C on a rotary shaker at 150 rpm. Mycelium was removed by filtration over Miracloth and the culture filtrate was extracted twice with an equal volume of ethyl acetate. After evaporation of the solvent under reduced pressure the residue was solved in 1 ml of methanol and subjected to HPLC analysis using a Dionex UltiMate3000 (ThermoFisher Scientific) and Eclipse XDB-C18 column, 5 μm, 4.6 × 150 mm (Agilent) that was kept at 40 °C. A binary solvent system consisting of water acidified with 0.1% formic acid (solvent A) and methanol (solvent B) was applied. The following gradient at a flow rate of 1 ml/min was used: 0–0.5 min 10% B, 0.5–15 min 10–90% B, 15–17 min 90% B, 17–17.5 min 90–100% B, 17.5–22 min 100% B, 22–23 min 100–10% B, 23–25 min 10% B. An authentic sample containing a mixture of aspulvinone E and isoaspulvinone E served as reference.
Semiquantitative RT-PCR analyses
To analyse transcription of genes from the polycistronic messenger RNA of the ATNT16 P2A_P2A strain RNA was isolated using the MasterPure-Yeast RNA Purification Kit (Epicentre) from mycelium cultivated for 24 h in the absence or presence of 15 µg/ml doxycycline or pre-grown for 18 h without doxycycline and further cultivated for 6 h after addition of doxycycline. After a DNase treatment (TURBO DNase; ThermoFisher) RNA was transcribed into cDNA as previously described . For normalisation of cDNA levels in the respective samples, serial dilutions were used for amplification of the A. niger actin gene using oligonucleotides 25 and 26. These primers span an intron region, which allows visualisation of a band shift from cDNA compared to genomic DNA (gDNA) and confirms the absence of contaminating gDNA in cDNA samples. For amplification of the melA gene oligonucleotides 15 and 16, for tyrP oligonucleotides 27 and 28 and for the tdTomato gene oligonucleotides 20 and 29 were used. PCRs of 30 cycles were performed in a SpeedCycler2 (Analytik Jena) using Phire Hot Start II polymerase (Thermo Scientific).
Fluorescence microscopy was performed as described previously  with some minor modifications. Strains were spotted on GG10 agar plates and pre-grown at 28 °C for one day, after which GG10 agarose coated coverslips containing 10 µg/ml doxycycline were placed next to the growing colony. 12 to 16 h later the coverslips were removed and placed on an object slide, overlaid with a droplet of mounting solution containing DAPI (ProLong Gold Antifade with DAPI, Thermo Scientific) and covered with a large coverslip. A GXML3201LED microscope (GX microscopes) was used for picture acquisition. Overlays of images were assembled by using the GIMP 2 software.
Comparison of expression levels from β-galactosidase activity determinations were analysed using GraphPad Prism (GraphPad Software) by applying multiple t-tests using the Holm-Sidak method, with α = 0.05. Each row was analysed individually, without assuming a consistent standard deviation.
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EG performed experiments and collected data. EG and MB designed the study, analysed data and wrote the manuscript. All authors read and approved the final manuscript.
We are grateful to Vera Meyer and Franziska Wanka (Technical University Berlin, Germany) for providing plasmid pFW22.1 containing the Tet-on promoter system.
Availability of data and materials
All main data generated or analysed during this study are included in this published article. Raw data on enzymatic activity determinations and pictures of Southern blots for selection of transformants are available from the corresponding author on reasonable request.
The authors declare that they have no competing interests.
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Additional file 1. Southern blot analyses and plasmid map of construct used for generation of ATNT strains. (A) Southern blot for identification of single copy integration strains. A digoxygenin labelled probe was used for hybridisation. Plasmid control and genomic DNA of parental strains and transformants were restricted with ApaI, which cuts once in the respective plasmid. The transformant used in subsequent analyses is numbered. (B) Plasmid map of the transformation construct. Position of oligonucleotides used in this study (P + number) as well as the position of the probe generated for Southern blot analysis and position of the restriction enzyme are shown. ble = phleomycin resistance cassette. TetOn = Tet-on promoter system. terR = terR gene including its native terminator sequence.
Additional file 2. Southern blot analyses and plasmid maps of constructs used for generation of lacZ reporter strains. (A, C) Southern blot for identification of single copy integration strains. Digoxygenin labelled probes were used for hybridisation. Transformants used in subsequent analyses are numbered. (A) A1144 strains with integration of the tet-on:lacZ construct. Plasmid control and genomic DNA of parental strains and transformants were restricted with AhdI, which cuts once in the respective plasmid. (C) ATNT16 strain transformed with the PterA:lacZ construct. Plasmid control and genomic DNA of parental strains and transformants were restricted with HindIII, which cuts once in the respective plasmid. (B, D) Plasmid maps of the transformation constructs. Position of oligonucleotides used in this study (P + number) as well as the position of the probe generated for Southern blot analyses and position of the restriction enzyme are shown. ptrA = pyrithiamine resistance cassette. hph = hygromycin resistance casette. PterA = terA promoter from Aspergillus terreus. lacZ = β-galactosidase gene from Escherichia coli. TtrpC = trpC terminator sequence from Aspergillus terreus.
Additional file 3. Southern blot analysis and plasmid map of construct used for generation of ATNT melA strains. (A) Southern blot for identification of single copy integration strains. A digoxygenin labelled probe was used for hybridisation. Plasmid control and genomic DNA of parental strains and transformants were restricted with BglII, which cuts once in the respective plasmid. The transformant used in subsequent analyses is numbered. (B) Plasmid map of the transformation construct. Position of oligonucleotides used in this study (P + number) as well as the position of the probe generated for Southern blot analysis and position of the restriction enzyme are shown. ptrA = pyrithiamine resistance cassette. PterA = terA promoter from Aspergillus terreus. TtrpC = trpC terminator sequence from Aspergillus terreus. melA = Aspulvinone E synthetase gene melA from Aspergillus terreus.
Additional file 4. Southern blot analyses and plasmid maps of constructs used for generation of ATNT16 P2A_P2A and P2A strains. (A) Southern blot for identification of single copy integration strains. A digoxygenin labelled probe was used for hybridisation. Plasmid control and genomic DNA of parental strains and transformants were restricted with XbaI, which cuts once in the respective plasmids. Transformants used in subsequent analyses are numbered. (B, C) Plasmid maps of the transformation constructs. Position of oligonucleotides used in this study (P + number) as well as the position of the probe generated for Southern blot analysis and position of the restriction enzyme are shown. hph = hygromycin B resistance cassette. PterA = terA promoter from Aspergillus terreus. TtrpC = trpC terminator sequence from Aspergillus terreus. melA = Aspulvinone E synthetase gene melA from Aspergillus terreus. tyrP = tyrosinase gene tyrP from Aspergillus terreus. tdTomato = codon optimised tdTomato gene. tyrP:tdTom = fusion of tyrP and tdTomato genes. P2A = sequence coding for the 2A peptide from porcine teschovirus-1.
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Geib, E., Brock, M. ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger . Fungal Biol Biotechnol 4, 13 (2017). https://doi.org/10.1186/s40694-017-0042-1