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Fig. 2 | Fungal Biology and Biotechnology

Fig. 2

From: ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger

Fig. 2

Heterologous expression of the aspulvinone E synthetase gene melA in the ATNT system. a Cultures of an ATNT16 melA OE strain cultivated with or without 15 µg/ml doxycycline and b ethyl acetate extraction of these cultures (top phase is ethyl acetate). c Extracts from (b) were dried and solved in 1 ml of methanol and 10 µl were subjected to HPLC analysis. The doxycycline induced culture confirms the biosynthesis of aspulvinone E (1) and its stereoisomer isoaspulvinone (2) as deduced from authentic standards

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