Fig. 3

Transcript levels of cbh1, xyn1 and xyr1 in absence and presence of Cre1-96. T. reesei strains Rut-C30 (blue bars) and both Rut-C30Δcre1-96 strains (yellow and orange bars) were cultivated in liquid medium supplemented with 1% (w/v) lactose for 36, 48 and 60 h. Transcript analyses of cbh1 (a), xyn1 (b) and xyr1 (c ) were performed in biological and technical duplicates by qPCR, data were normalized to the housekeeping genes sar1 and act, and referred to the respective transcript levels of Rut-C30 at 36 h. The relative transcript ratios are given as means and the error bars indicate the standard deviations. All values were statistically analysed in a confidence interval of 95% and asterisks indicate significant differences