Fig. 3

Enzymatic digestion of the cellulose substrates in vitro. All celluloses were digested by a N. crassa-derived cellulase cocktail (filtered culture supernatant after 5 days growth on Avicel) for a total of 24 h. a Shown are representative HPAEC-PAD chromatograms of the reaction supernatants after 8 h as well as the quantification results for monosaccharides at an initial time point (1 h; inset in a ). The peaks of d-glucose (d-Glc) and higher cellodextrins (not quantified) are indicated. Note: in this run (CarboPac^® PA200 column), the other monosaccharides will also migrate at the same speed as d-Glc, but the amounts are substantially lower. The quantifications represent means of triplicate reactions. The error bars represent standard deviations. Letters indicate data groups that are significantly different (one-way ANOVA, p-values < 0.05 were considered significant). b–m Representative scanning electron micrographs out of technical triplicates for each cellulose substrate obtained at 8.0 kV accelerating voltage and × 30–× 2000 magnification (Jeol JSM-IT100)