Fig. 3

Fluorescent microscopic quantification of chitin in lateral cell wall and septa during growth on solid agar. Strains were grown on MM plates for 48 h at 20 ℃. Chitin was measured using Calcofluor White staining and fluorescent signal normalized to that of the control strain (A). Statistically significant changes relative to control are indicated (t-tests). Exemplar images are shown for MJK17.25 control and two mutants isolates (B). Scale bar = 5 µm