Fig. 2

Deletion of chitin synthase encoding genes has diverse impacts on A. niger growth in solid and liquid cultivation. Light microscopic analysis of (A) germlings following 12 h incubation in CM liquid medium at 30°C without shaking or (B) young colonies following 26 h growth on MM agar at 30 ℃. Scale bar = 50 µm. Pellets grown in liquid culture were analysed as slices of 100 µm thickness using the µCT approach (C). Scale bar = 100 µm. Colony images (D) were captured following 6 days growth at 30 ℃ on MM. White values in upper section of each respective image are colony area normalized to the MJK17.25 control. White values in bottom of each colony image are spore titres normalized to colony area and reported as a percent of MJK17.25. Standard deviation from triplicate replicates is indicated