Fig. 9
From: Non-canonical two-step biosynthesis of anti-oomycete indole alkaloids in Kickxellales
Thiotemplated amidation reactions in microbes. The thiotemplated amidation in secondary metabolism requires an active thioester prior to peptide transfer. The donor acid is usually bound to a carrier protein (CP) that is either part of an NRPS (A) or a freestanding CP domain protein (B). In contrast, lindolin biosynthesis in Kickxellomycetes rely on a CP-independent but CoA-mediated amide formation (C). A adenylation domain, ACP acyl carrier protein, C condensation domain, NatC1/2 freestanding CPs, NatL1/2 aryl-AMP synthetases, NatS aryl-ACP synthases, T thiolation domain (NRPS-bound CP). Donor and acceptor acids are highlighted in blue and red, respectively