Fig. 6

Biocatalytic lindolin production in vitro. A Schematic representation of the two-step biosynthesis to lindolins. The reaction was initiated by addition of the substrate anthranilic acid (AA) (B and C) or 5-hydroxyanthranilic acid (HAA) (D) and incubated at 25 °C for 2 h. The amount of 4 (B), IAA-CoA (C) and 5 (D) was chromatographically quantified. Representative EICs are given for 4, IAA-CoA and 5 at m/z 293 [M−H]⁻, m/z 923 [M−H]⁻, and m/z 309 [M−H]⁻. Note, that the CoA ester leads to slightly broadened peaks as described elsewhere [51]. E Enzyme-coupled determination of LinA activity in presence and absence of LinB