Fig. 9
From: Bifurcate evolution of quinone synthetases in basidiomycetes
HPLC–MS analysis of mutated quinone synthetases. Shown are extracted ion chromatograms (EICs). A Chromatograms of polyporic acid and atromentin standards, chromatograms of ethyl acetate extracts of the culture broth of A. nidulans expressing corA, ppaA1, nps3, or mutated corA, ppaA1 or nps3 variants. As controls, extracts of the untransformed A. nidulans parental strain and empty vector control strain A. nidulans tPS15 are shown. B Mass spectra of chromatographic signals of the tPS36 extract, recorded in negative mode, match the calculated masses of atromentin and polyporic acid (m/z 323.0561 and m/z 291.0663 [M-H].−). MS/MS data are shown in Additional file 2: Fig. S21