Fig. 5

Product formation by wild type and mutated Psilocybe cubensis quinone synthetase PpaA1, analyzed by UHPLC-MS. Analyzed peaks are indicated by numbered bars shaded in grey. Chromatograms were extracted at λ = 350 nm. A Upper panel: chromatograms of synthetic polyporic acid (3) and of authentic phlebiopsin B (2). Center panel: chromatograms of ethyl acetate extracts of the culture broth of Aspergillus niger expressing ppaA1 or mutated ppaA1 variants. Bottom panel: chromatograms of ethyl acetate extracts of the culture broth of Aspergillus nidulans expressing ppaA1 or mutated ppaA1 variant. For control, extracts of untransformed parental strains and empty vector control strains A. niger tNAL000 and A. nidulans tPS15 are shown. The background conversion of added polyporic acid to phlebiopsins (phlebiopsin A (1)) by the latter two control strains is shown as well. B Mass spectra of chromatographic signals of the tPS11 extract, recorded in negative mode. UV/Vis spectra are shown in Additional file 2: Fig. S4, HR-MS and MS/MS data in Additional file 1: Table S3, Additional file 2: Figs. S11 and S13. Additional chromatograms are shown in Additional file 2: Fig. S12