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Table 2 Number of protoplasts prepared with driselase and lysing enzymes in MgSO4-buffer (pH 5.8)

From: Stable overexpression and targeted gene deletion of the causative agent of ash dieback Hymenoscyphus fraxineus

 

Experiment 1

Experiment 2

Experiment 3

Driselase

7.5 × 105

8.4 × 105

8.0 × 105

Lysing enzymes

4.2 × 105

4.6 × 105

2.4 × 105