Fig. 7

Verification of the replacement of the hygromycin resistance by a geneticin resistance in mutant #1.12 via homologous recombination. A Plate assay. Mutant #25.7 grew on both selectable mediums, mutant #1.12 grew on AM_HygS but not on AM_GenS while mutant #25.8 grew on AM_GenS but not on AM_HygS. Scale bar = 1 cm. B PCR to detect the hph gene (expected band size is 452 bp) and the nptII gene (expected band size is 421 bp). In mutant #1.12 only a fragment of the hph gene and in mutant #25.8 only a fragment of the nptII gene was detected. In mutant #25.7, both genes were detected. M = GeneRuler^TM1kb Plus DNA Ladder (ThermoFisher, Waltham, USA). Sizes of the marker bands are displayed in bp at the right. C_Hyg = positive control pAN_HygmCherry, C_Gen = positive control pIGPAPA_GenGFP