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Fig. 1 | Fungal Biology and Biotechnology

Fig. 1

From: Stable overexpression and targeted gene deletion of the causative agent of ash dieback Hymenoscyphus fraxineus

Fig. 1

Drawing of the strategy for the replacement of the hph gene of mutant #1.12 by the nptII gene. A The final construct with the nptII resistance cassette (pIGPAPAGenGFP) and B clipped part of the pIGPAPAHygGFP. Flanking regions are indicated as crossed boldface black lines. Prior to transformation, the construct was excised PvuI from the plasmid pIGPAPAGenGFP

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