Table 1 Components of the digestive EV treatments for eliminating intravesicular and apoplastic co-purified proteins and RNAs
Group | TE sprayed barley/wt A. thaliana | dsCYP3RNA sprayed barley/A. thaliana CYP3RNA | ||||
|---|---|---|---|---|---|---|
1 | 2 | 3 | 1 | 2 | 3 | |
EV solution | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl |
RNase | – | 1.2 µl | 1.2 µl | – | 1.2 µl | 1.2 µl |
PK | – | 3 µl | 3 µl | – | 3 µl | 3 µl |
Triton X | – | – | 5.8 µl | – | – | 5.8 µl |
PBS | 10 µl | 5.8 µl | – | 10 µl | 5.8 µl | – |
Total | 60 µl | 60 µl | 60 µl | 60 µl | 60 µl | 60 µl |