Fig. 8

Structural model of the monooxygenase MO6277. a Alphafold structure model of MO6277 depicting a single membrane-spanning N-terminal alpha-helix and a buried haem binding site (docked haem model shown in orange van der Waals spheres). The potential location of the membrane surface is depicted as grey line. Secondary structure elements are coloured by sequence (BGR). b Substrate tunnels to the haem-containing binding site as calculated using CAVER software. Tunnels are coloured by priority as determined by the narrowest bottleneck from green (high priority) to red (low priority). c The most prominent tunnels to the active site from the membrane (blue) and cytosol facing side (orange)