Fig. 5
From: Characterisation of ascocorynin biosynthesis in the purple jellydisc fungus Ascocoryne sarcoides
Purification of recombinant AcyN and in vitro characterisation of metabolite production. a SDS-PAGE analysis of Strep-tactin purified AcyN from A. oryzae OP12. CFE = cell free extract, FT = column flow through, Wash = column wash fraction, Elu = elution fraction. b HPLC analysis of extracts from the acid-precipitated pellet fractions from in vitro assays with purified AcyN. Numbers in brackets denote the concentration of substrates in [mM]. P, phenylpyruvate, p-OH-PP, p-hydroxphenylpyruvate, Cont, control reaction in the absence of AcyN. Positions of standards are indicated by grey bars. For the analysis of the supernatant extracts from in vitro reaction refer to Additional file 1: Fig. S1