Fig. 3

DCS12-AmB-LLs more efficiently inhibited or killed three diverse fungal species in vitro than un-targeted control liposomes. In vitro grown fungal cells were treated with DCS12-AmB-LLs, BSA-AmB-LLs or AmB-LLs delivering the indicated concentrations of AmB or with liposome dilution buffer. a C. albicans hyphal cells growing in RPMI in microtiter plates were treated with liposomes delivering 0.1, 0.05 and 0.025 µM AmB. Metabolic activity was measured as relative fluorescence units (RFU) generated from reduction of the CellTiter-Blue reagent. b A. fumigatus hyphal cells growing in RPMI in microtiter plates were treated with liposomes delivering 0.5, 0.25 and 0.125 µM AmB. Metabolic activity was measured with CellTiter-Blue reagent. c C. neoformans yeast cells growing in liquid YPD suspension were treated in with liposomes delivering 0.5, 0.25 and 0.125 µM AmB and surviving cells assayed as CFUs. N = 5 or N = 6 for each bar. Scatter bar plots indicate the inhibition and/or killing of fungal cells. The fold-difference in RFUs or CFUs and P or P_MW values are shown for informative comparisons