Fig. 1

Schematic maps of single guide RNA (sgRNA) expression constructs and the Fusarium venenatum PKS12 gene indicating the target site used for CRISPR/Cas9 editing. A Fv5SrRNA promoter-sgRNA construct, showing positions of PFv5SrRNA, 20 base target site sequence (yellow and black striped box), scaffold sequence (Sc) and terminator (T). B PgdpA dual ribozyme-sgRNA construct showing positions of PgdpA, inverted repeat (blue arrow) of first six bases in target sequence (yellow and black box), HH and HDV ribozyme sequences and trpC terminator (TtrpC). C F. venenatum PKS12 gene showing exons (yellow arrows) and the location of the CRISPR target sequence (pink arrow) in exon 3, 3-14 (‘14’) antisense strand, sequence position 3’–5’ = 238-257