Fig. 2
From: An overview on current molecular tools for heterologous gene expression in Trichoderma
Agrobacterium tumefaciens-mediated transformation. The native Ti plasmid contains the vir region and the T-DNA that is flanked by left and right borders (LB, RB). The T-DNA can be replaced by a gene of interest (GOI) and a selection marker (SM) by cloning in E. coli. The recombinant plasmid is then transformed into A. tumefaciens. Upon stimulation with acetosyringone, the VirA/C histidine kinase receptor actives expression of the vir genes, which result in release and transformation of the T-DNA (in complex with VirE2 and VirD2) into the host cell and nuclear import