Fig. 1

Schematic overview of the aspects to be considered for heterologous gene expression. First, the DNA containing the expression cassette needs to be transformed (1) into the fungal cell. Suitable (ideally reusable) markers (2 + 3) are used to select for ectopic integration or targeted insertion (4 + 5), which might be supported by the CRISRP/Cas9 system (6). For optimal expression, the choice of promoters (7), potentially 2A peptides for polycistronic expression (8), codon-optimization (9), and terminators (10) need to be considered. If a protein is to be secreted (11) a signal peptide needs to be added