Table 1 T. reesei strains used in this study

QM6a Δpyr4

Wild-type-like strain with deficiency of the non-homologous end joining repair pathway, and uridine auxotrophy

[10]

QM6a Δhis1 (pyrG  +)

Histidine auxotrophic strain, obtained through transformation of QM6a Δpyr4 with pCD-Δhis1, carries the A. fumigatus pyrG gene

This study

QM6a Δpyr4 Δhis1

Uridine and histidine auxotrophic strain, obtained through pyrG excision in QM6a Δhis1 (pyrG  +)

This study

QM6a Δpyr4 eyfp (his1)

EYFP-reporter strain, expression cassette integrated at this his1 locus, obtained through transformation of QM6a Δpyr4 Δhis1 with pCD-ReHis-eyfp

This study

QM6a eyfp (pyr4)

EYFP-reporter strain, expression cassette integrated at this pyr4 locus

[10]

QM6a eyfp (asl1)

EYFP-reporter strain, expression cassette integrated at this asl1 locus

[10]

QM6a Δpyr4 Δhis1 Δasl1

Uridine, histidine, and arginine auxotrophic strain, obtained through transformation of QM6a Δpyr4 Δhis1 with pCD-Δasl1, recipient strain for multiple gene integrations

This study