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Table 3 Transformations of C. cinerea FA2222 (trp1.1,1.6) with plasmid pBD5 alone or, using same batches of protoplasts, in combination with other non-directly selectable vectors

From: Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut of Coprinopsis cinerea

Plasmid(s) trp1+-selected transformants collected on Total transformants Ratio of clones
1st day 2nd day 3rd day 4th day 5th day
Experiment 1
pBD5 13 8 12 3 2 38 1.0
pBD5 + pYSK7* 30 (8) 20 (13) 32 (7) 9 (2) 4 (2) 95 (32) 2.5
pBD5 + pDB3 32 13 25 6 3 79 2.1
pBD5 + pPAB1-2 18 17 17 7 2 61 1.6
pBD5 + pCcAde8 34 27 11 4 2 78 2.1
Experiment 2
pBD5 46 38 28 12 11 135 1.0
pBD5 + pYSK7* 94 (31) 89 (22) 68 (27) 15 (12) 5 (3) 271 (95) 2.0
pBD5 + pDB3 69 52 53 15 12 201 1.5
pBD5 + pPAB1-2 76 78 49 28 14 245 1.8
pBD5 + pCcAde8 100 114 90 26 14 344 2.5
  1. *Date in brackets indicate clones expressing laccases as deduced from stained halos around their colonies. Non-producers of laccase did not stain the agar. Random subsets of unstained pBD5 and of staining pBD5 + pYSK7 clones from both experiments were further tested in liquid fermentations
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