Fig. 1

A heterogeneous population of EVs is secreted by Z. tritici in vitro. a, b are representative of several biological replicate EV samples, with (a) showing representative TEM data of 2% uranyl acetate-stained EV samples. Images were cropped and scale bars added using ImageJ; no other modifications were made. b Boxplot showing the distribution in EV diameter, measured using ImageJ of the representative EV sample, shown in a. The median particle size was 84.0 nm; the cross represents a mean particle size of (91.83 nm ± 39.9 sd). Each point on the boxplot represents an EV-like particle from the TEM data (n = 649). c NTA data showing particle size versus concentration of particles per mL of fungal culture for 3 biological replicates. The size distributions were averaged across 3 technical replicates and the standard error for each plotted value is indicated with shading. Mean particle size ranged from 168.0–195.2 nm and the modal particle size from 134.13 to 150.3 nm