Table 2 Primers used in this study
Name | Sequence (5′-3′) |
|---|---|
Ypr1_L185f-VspI | ATTAATCTTACTCCACAGTCGACAACG |
Ypr1_G674r-MfeI | CAATTGCGCCGTAAATGCTCCCATCG |
5pyr4_fwd3 | CCAGACGGTGATTCACATATACG |
Ptef_rev-BspTI | CTTAAGTGTGATGTAGCGTGAGAGCTG |
pyr4_3fwd | AGACGAGGACCAGCAGACC |
Tpyr4_rev2 | CAGGAAGCTCAGCGTCGAG |
5pyr4_fwd(BglII) | GCGGAAGATCTCGAGATAGTATCTC |
5pyr4_rev-BspEI | TCCGGAGTAGCTCTTCACTGGTTGTGGTG |
sar1fw | TGGATCGTCAACTGGTTCTACGA |
sar1rev | GCATGTGTAGCAACGTGGTCTTT |
act1f | TGAGAGCGGTGGTATCCACG |
act1r | GGTACCACCAGACATGACAATGTTG |
cbh1f | GATGATGACTACGCCAACATGCTG |
cbh1r | ACGGCACCGGGTGTGG |
cbh2f | CTATGCCGGACAGTTTGTGGTG |
cbh2r | GTCAGGCTCAATAACCAGGAGG |
egl1f | CTGCAACGAGATGGATATCCTGG |
egl1r | GTAGTAGCTTTTGTAGCCGCTGC |
xyn1f | CAGCTATTCGCCTTCCAACAC |
xyn1r | CAAAGTTGATGGGAGCAGAAG |
xyn2_q1f | CCGTCAACTGGTCCAACTCG |
xyn2_q1r | GTGCGGTAAATGTCGTAGACG |
xyl1-fwd | CTGTGACTATGGCAACGAAAAGGAG |
xyl1-rev | CACAGCTTGGACACGATGAAGAG |