Fig. 2

Construction and validation of eYFP overexpression mutant Fs OE::eYFP-6. a Integration of pSHUT4-eYFP T-DNA cassette into a non-coding position of the F. solani genome through homologous recombination between identical segments (blue). Primer positions are marked, and a full list of primers is found in Additional file 1. b DIC pictures and EFI projections comparing parental and mutant strain phenotypes. Scale bar = 100 µm. c Genome sequencing validation displays correct integration of the pSHUT4-eYFP T-DNA cassette via homologous recombination. d Growth and eYFP induction of F. solani wild type and OE::eYFP-6 during growth on nine different media. The left panel illustrates growth (optical density) over a 7 days period. The right panel illustrates fluorescence at excitation/emission wavelength of 514/526. All data points are the average of four biological replicates, expressed as a percentage of the maximum observed value