Table 1 Strains used in this study

AB1.13

AB1.13 WT

Uridine auxotroph [14]

AB1.13 pyrG+

AB1.13

Uridine prototroph of AB1.13 [15]

AB1.13 CAD 4.1

AB1.13 CAD

Selected pyrG+ transformant of cadA expressing transformant (CAD10.1) of AB1.13 [16]

AB1.13 CAD + MFS + MTT #49B;

AB1.13 #49B

Selected mttA expressing transformants of AB1.13 CAD + MFS 3.9 [8]

AB1.13 CAD + MFS + MTT + CitB #99

CitB#99

Selected citB overexpressing strain of AB1.13 CAD + MFS + MTT #49B [8]

AB1.13 cimA A10

AB1.13 cimA B3

AB1.13 cimA D11

CimA A10

CimA B3

CimA D11

Selected cimA overexpressing strain of AB1.13 (this study)

AB1.13 cimA B3 pyrE−

CimA B3 pyrE−

pyrE mutant strain of CimA #B3 (this study)

AB1.13 cimA B3 + mfsB 27

CimA + MfsB #27

Selected mfsB overexpressing strain of AB1.13 cimA B3 pyrE− (this study)

AB1.13 cimA B3 + mfsB 28

CimA + MfsB #28