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Table 4 Percentage content of HMF, DFF, FFCA and FDCA in the cascade reactions of UmaAAO and PciGLOX3 following condition (1) that consisted of reacting UmaAAO with HMF for 2 h before adding PciGLOX3 and condition (2) in which PciGLOX3 was added after 24 h of the reaction of UmaAAO with HMF

From: Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives

 

Condition (1)

Condition (2)

After 4 h

After 24 h

After 4 h

After 24 h

NC

C

NC

C

NC

C

NC

C

HMF

ND

NDa

ND

ND

ND

ND

ND

ND

DFF

17% ± 3.3

ND

1% ± 0.3

ND

ND

ND

ND

ND

FFCA

83% ± 1.2

96% ± 0.1

98% ± 0.7

94% ± 0.1

90% ± 6.2

87% ± 5.3

86% ± 0.4

84% ± 1

FDCA

ND

4% ± 0.8

1% ± 0.03

6% ± 0.03

10% ± 0.2

13% ± 0.6

14% ± 0.1

16% ± 1.2

  1. Reactions were performed in the presence (C) and absence of catalase (NC) for both conditions. PciGLOX3 was reacted for 4 or 24 h before analyzing the reaction products. Standard deviations are presented as plus-minus values
  2. aND, values below 1%
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Fungal Biology and Biotechnology

ISSN: 2054-3085

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