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Table 4 Percentage content of HMF, DFF, FFCA and FDCA in the cascade reactions of UmaAAO and PciGLOX3 following condition (1) that consisted of reacting UmaAAO with HMF for 2 h before adding PciGLOX3 and condition (2) in which PciGLOX3 was added after 24 h of the reaction of UmaAAO with HMF

From: Pycnoporus cinnabarinus glyoxal oxidases display differential catalytic efficiencies on 5-hydroxymethylfurfural and its oxidized derivatives

  Condition (1) Condition (2)
After 4 h After 24 h After 4 h After 24 h
NC C NC C NC C NC C
HMF ND NDa ND ND ND ND ND ND
DFF 17% ± 3.3 ND 1% ± 0.3 ND ND ND ND ND
FFCA 83% ± 1.2 96% ± 0.1 98% ± 0.7 94% ± 0.1 90% ± 6.2 87% ± 5.3 86% ± 0.4 84% ± 1
FDCA ND 4% ± 0.8 1% ± 0.03 6% ± 0.03 10% ± 0.2 13% ± 0.6 14% ± 0.1 16% ± 1.2
  1. Reactions were performed in the presence (C) and absence of catalase (NC) for both conditions. PciGLOX3 was reacted for 4 or 24 h before analyzing the reaction products. Standard deviations are presented as plus-minus values
  2. aND, values below 1%