Fig. 3
Schematic presentation of the a workflow and b target genes for multiplexed A. niger genome editing using the microtiter plate scale CRISPR/Cas9 method. The workflow was demonstrated by engineering the fungal d-galacturonate pathway for galactarate (mucic acid) production. The competing pathway genes gaaA, encoding a d-galacturonate reductase, gaaC, encoding an l-galactonate dehydratase, and 39114, encoding an enzyme involved in galactarate catabolism, and gaaX, encoding a repressor protein of pectin catabolic pathways, were deleted by replacing the genes with DNA cassettes expressing the bacterial uronate dehydrogenase (UDH)