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Fig. 3 | Fungal Biology and Biotechnology

Fig. 3

From: Translocated duplication of a targeted chromosomal segment enhances gene expression at the duplicated site and results in phenotypic changes in Aspergillus oryzae

Fig. 3

a Confirmation of translocated duplication by PCR. Targeted translocated duplication of a 1.4-Mb region of chromosome 2 to chromosome 4 was confirmed by PCR. Lane 1. B–U, B–L; Lane 2. ct166-U, ct166-L; Lane 3. B–U, ct166-L; Lane 4. ct166-U, B–L (Lanes 1–4. translocated duplication strain J4); Lane 5. B–U, B–L; Lane 6. ct166-U, ct166-L; Lane 7. B–U, ct166-L; Lane 8. ct166-U, B–L (Lanes 5–8. Wild-type strain). b Confirmation of translocated duplication by comparative genome hybridization (CGH) arrays. Vertical bars show ratios of signal intensities for probes of the strain bearing translocated duplication (J4) relative to those of the control strain (RIB40). Ratios of hybridization signals in the 1.4-Mb region of chromosome 2 were twice those of the other region, indicating that the 1380-kb region of chromosome 2, including AO090002001035 to AO090002001558, was duplicated. The ratio of hybridization signals in the 308-kb region of chromosome 4 was nearly zero, indicating that the 308-kb region of chromosome 4, including AO090166000010 to AO090166000123, was deleted

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