Fig. 4
CNB treated RSY150 C. albicans exhibit a cell wall stress response. Logarithmic phase RSY150 cells were treated with 1/2 and 1/4 MIC CNB oil or without oil for 24 h. a Treated and control cells at 105, 104 and 103 CFU were spotted on YNB complete media agar containing CFW and CR and examined after 2 days. Treated cells showed greater sensitivity to cell wall stressors. b The number of colonies on the CR and CFW plates show a dose dependent effect that decreases as a function of oil concentration. The 1/2 MIC CNB oil was more effective than 1/4 MIC, with a significant difference ** between 1/2 MIC oil treatment compared to untreated controls (p < 0.05). The cell count was normalized with treated cells grown on YNB media agar only. c Wild type background strain (CaSS1) and cell wall defective mutants (SSU81, CRH, DFG and VPS) were exposed to a twofold increasing dilution of CNB oil in a MIC assay, showing overall sensitivity to the CNB oil and indicating a moderate cell wall stress response