Fig. 3
Surface remodelling of RSY150 C. albicans exposed to 1/2 MIC CNB oil. RSY150 and the clinical isolate from blood were treated with 1/2 MIC (62.5 μg/mL) CNB oil for 24 h, deposited and fixed on PLL-coated cover slips for AFM imaging. Images (1 μm; 128 px2) of control and CNB oil treated cells are at low resolution for control (a, c) and treated (b, d) C. albicans showing leakage (arrow heads) in treated cells. High resolution (1 μm) height images of the cell surface (boxed area) for control (e, g) and CNB treated (f, h) C. albicans reveal increased surface roughness in the treated clinical isolate as compared to the treated RSY150 strain. (i, j, k, l) High resolution (1 μm) adhesion maps from corresponding height images. Bar a–d = 1 μm; Bar e–l = 200 nm