Table 1 Strains of Leptosphaeria spp. used in this study
Strain name(s) | Genotype | Origin |
|---|---|---|
L. biglobosa 06J154 | Wild type | Burren Junction, NSW, Australia, 2006 [19] |
D5 (IBCN18; M1) | Wild type | Penshurst, VIC, Australia, 1988 |
D5-IpR | hos1 − | Selection of D5 on iprodione |
D5-IpR + hos1 | hos1 − + hos1 | Transformation of D5-IpR with wild type hos1 |
D2 (IBCN15) | Wild type | Streatham, VIC, Australia, 1988 [2] |
D2-IpR | hos1 − | Selection of D2 on iprodione |
D2-IpR + hos1 | hos1 − + hos1 | Transformation of D2-IpR with wild type hos1 |
D3 (IBCN16) | Wild type | Mt Barker, WA, Australia, 1988 [2] |
D3-IpR | hos1 − | Selection of D3 on iprodione |
D3-IpR + hos1 | hos1 − + hos1 | Transformation of D3-IpR with wild type hos1 |
14P290 | Wild type | Katanning, WA, Australia, 2014 [17] |
14P290-IpR | hos1 − | Selection of 14P290 on iprodione |
14P290-IpR + hos1 | hos1 − + hos1 | Transformation of 14P290-IpR with wild type hos1 |
D13 (09SMW024) | Wild type | Cummins, SA, Australia, 2009 [52] |
DV1 | hos1 guide | D5 |
DV2 | hos1 guide; cas9 | DV1 |
DV3 | hos1 guide; cas9; hos1 − | DV2 |
D13-CoT | cas9; hos1 guide | D13 cotranformed with both guide RNA (hyg) and Cas9 (G418) |
D13-IpR1 | cas9; hos1 guide; hos1 − | Selection of D13-CoT on iprodione |
D13-IpR2 | cas9; hos1 guide; hos1 − | Selection of D13-CoT on iprodione |
v23.1.3 | Wild type | In vitro crosses, France [5] |
JN3-Cas9 | cas9 | v23.1.3 transformed with pMAI23 |
JN3-avrLm1-1 | cas9; AvrLm1 guide; avrLm1 − | JN3-Cas9 |
JN3-avrLm1-2 | cas9; AvrLm1 guide; avrLm1 − | JN3-Cas9 |