Table 1 Strains used and constructed within this work
From: Promoters from the itaconate cluster of Ustilago maydis are induced by nitrogen depletion
Name | Genotype | Origin |
|---|---|---|
Wildtype strains | ||
U. maydis MB215 | Wildtype | DSM 17144 |
E. coli DH5α | Wildtype | DSM 6897 |
Recombinant U. maydis strains | ||
U. maydis MB215 P otef -gfp (s) | Single integration of pUMa43-otef-gfp-nos cbx | This work |
U. maydis MB215 P otef -gfp (m) | Multiple integration of pUMa43-otef-gfp-nos cbx | This work |
U. maydis MB215 P tad1 -gfp (s) | Single integration of pUMa43-P tad1 -gfp cbx | This work |
U. maydis MB215 P tad1 -gfp (m) | Multiple integration of pUMa43-P tad1 -gfp cbx | This work |
U. maydis MB215 P itp1 -gfp (s) | Single integration of pUMa43-P itp1 -gfp cbx | This work |
U. maydis MB215 P itp1 -gfp(m) | Multiple integration of pUMa43-P itp1 -gfp cbx | This work |
U. maydis MB215 P adi1 -gfp (s) | Single integration of pUMa43-P adi1 -gfp cbx | This work |
U. maydis MB215 P adi1 -gfp (m) | Multiple integration of pUMa43-P adi1 -gfp cbx | This work |
U. maydis MB215 P mtt1 -gfp (s) | Single integration of pUMa43-P mtt1 -gfp cbx | This work |
U. maydis MB215 P mtt1 -gfp (m) | Multiple integration of pUMa43-P mtt1 -gfp cbx | This work |