Skip to main content

Table 1 Strains used in this study

From: Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans

Name Genotype Gene(s) affected Description Source
NID1 argB2, pyrG89, veA1, nkuAΔ nkuAΔ for efficient gene targeting Parental strain used to construct mRFP secreting strain IBT collection #29539
[33]
NID3 argB2, pyrG89, veA1, nkuA-trS::AFpyrG Transient small repeat in nkuA Reference strain IBT collection #28738
[33]
NID912 argB2, pyrG89, veA1, nkuAΔ, IS1::PgpdA::RFP::TtrpC::pyrG Intracellular mRFP expression Negative control Our lab
NID1439 argB2, pyrG89, veA1, nkuAΔ, IS1::PgpdA-ASNglaA-mRFP-TtrpC::AFpyrG mRFP secretion Strain secreting mRFP This study
NID1595 argB2, pyrG89, veA1, nkuAΔ, IS1::PgpdA-ASNglaA-mRFP-TtrpC mRFP secretion Strain secreting mRFP, parental strain for NID1596-NID1609 This study
NID1596 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN7679-TtrpC::AFpyrG mRFP secretion, AN7679 overexpressed Sc ERV41 ortholog This study
NID1597 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN2738-TtrpC::AFpyrG mRFP secretion, AN2738 overexpressed Sc ERV46 ortholog This study
NID1598 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN10724-TtrpC::AFpyrG mRFP secretion, AN10724 overexpressed Sc YIP3 ortholog This study
NID1599 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN11900-TtrpC::AFpyrG mRFP secretion, AN11900 overexpressed Sc BOS1 orholog This study
NID1600 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN0834-TtrpC::AFpyrG mRFP secretion, AN0834 overexpressed AN sec63 This study
NID1601 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN7302-TtrpC::AFpyrG mRFP secretion, AN7302 overexpressed Sc EMP47 ortholog This study
NID1602 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANRabD-TtrpC::AFpyrG mRFP secretion, ANrabD overexpressed AN rabD This study
NID1603 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANRabE-TtrpC::AFpyrG mRFP secretion, ANrabE overexpressed AN rabE This study
NID1604 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANSynA-TtrpC::AFpyrG mRFP secretion, ANsynA overexpressed AN synA This study
NID1605 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANSsoA-TtrpC::AFpyrG mRFP secretion, ANssoA overexpressed AN ssoA This study
NID1606 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-ANNsfA-TtrpC::AFpyrG mRFP secretion, ANnsfA overexpressed AN nsfA This study
NID1607 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN4759-TtrpC::AFpyrG mRFP secretion, AN4759 overexpressed Sc SEC2 ortholog This study
NID1608 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN10354-TtrpC::AFpyrG mRFP secretion, AN10354 overexpressed S.c. SEC11 ortholog This study
NID1609 argB2, pyrG89, veA1, nkuAΔ, IS5::PgpdA-ASNglaA-mRFP-TtrpC, IS1::PtetON-AN6307-TtrpC::AFpyrG mRFP secretion, AN6307 overexpressed An02g04250 ortholog This study
  1. AN, Aspergillus nidulans; An, Aspergillus niger; AF, Aspergillus fumigatus; Sc, Saccharomyces cerevisiae