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Table 2 Fungal strains used in this study

From: Phytochelatin synthase is required for tolerating metal toxicity in a basidiomycete yeast and is a conserved factor involved in metal homeostasis in fungi

Name

Genotype

Parent/origin

Reference

Sporobolomyces sp.

IAM 13481

Wild type

 

[35]

AIS2

ura5

IAM 13481

[49]

AS1

pcs1::URA5 ura5

AIS2

This study

AS2

pcs1::ura5 ura5

AS1

This study

AS3

pcs1::ura5 ura5 + PCS1-URA5

AS2

This study

Saccharomyces cerevisiae

BY4743

MAT a/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0

 

[57]

yap1

yap1::KanMX

BY4743

Open Biosystems

AS4

+ pYES2

BY4743

This study

AS5

+ pAS1

BY4743

This study

AS6

+ pYES2 yap1::KanMX

yap1

This study

AS7

+ pAS1 yap1::KanMX

yap1

This study

Schizosaccharomyces pombe

L972

Wild type

 

NBRP, Japan

MM72-4A

ura4-D18

 

NBRP, Japan

AS8

pcs1::KanMX ura4

MM72-4A

This study

AS9

+ pREP42

MM72-4A

This study

AS10

+ S. pombe PCS1 (pAS4)

MM72-4A

This study

AS11

+ Sporobolomyces PCS1 (pAS5)

MM72-4A

This study

AS12

pcs1::KanMX + pREP42

AS8

This study

AS13

pcs1::KanMX + S. pombe PCS1 (pAS4)

AS8

This study

AS14

pcs1::KanMX + Sporobolomyces PCS1 (pAS5)

AS8

This study

AS15

pcs1::KanMX + PCS1-GFP (pAS6)

AS8

This study

  1. The S. cerevisiae genotypes are abbreviated after their first use in strain BY4743.