Figure 3
The Sporobolomyces PCS1 gene complements the S. pombe pcs1 Δ phenotypes. (A) Ten fold serial dilutions of cultures of S. pombe strains on YPD with or without 10 μM CdSO4, and cultured 3 d at 30°C. The wild type or pcs1 deletion mutant strains contain the empty pREP42 plasmid or this plasmid including the cDNA clones of the PCS1 homologs from S. pombe or Sporobolomyces. (B) The pcs1 mutant has slower growth on YES + uracil (20 mg/L) + CuSO4 (0.625 mM) and an alteration in pigmentation. Growth of strains MM72-4A and AS8 after 6 d at 22°C.