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Table 2 Primers used in this study

From: A new vector for efficient gene targeting to the pyrG locus in Aspergillus niger

Primer name Sequence 5’-3’ Used for
ABpyrGP12for-EcoRI-AscI CGGAATTCGG CGCGCCCGGC TGACGTTACC ACCACT* pyrG-3’ UTR PCR (Figure 2A)
ABpyrGP10rev-NotI AAGGAAAAAA GCGGCCGCAG TCAGACCTAA TGCCTCGGG pyrG-3’ UTR PCR (Figure 2A)
ABpyrGP11for-NotI AAGGAAAAAA GCGGCCGCCG TCGCGTGATA AGGGTTG 3’ pyrG PCR (Figure 2A)
ABpyrGP13rev-SacI-AscI CGAGCTCGGC GCGCCTCGGG TCAATTTCCT CTGTTG 3’ pyrG PCR (Figure 2a)
SL1 ATTTGCGGCC GCAGAACGCC GAGAAGAACT GG PgpdA-mluc PCR (Figure 2A)
TtrpCP2rev-NotI AAGGAAAAAA GCGGCCGCTC TAGAAAGAAG GATTACCTC PgpdA-mluc PCR (Figure 2A)
  1. *Relevant restriction sites are shown in italic.