Figure 4
Dot blot assay to detect the presence of Gal f residues on secreted glycoconjugates of different A. niger strains. Strains were grown to early stationary phase and cell-free medium was spotted in a dilution series as indicated on nitrocellulose filter paper on two blots in parallel. The left blot was incubated with the anti-Galf antibody (L10) the right blot was incubated with the ConA lectin.