Figure 1

Dot blot assay to detect the presence of Gal f residues on secreted glycoconjugates from A. niger mutants. 240 A. niger cell wall mutant strains were grown to early stationary phase and cell-free medium was spotted on nitrocellulose filter paper. The blots were incubated with the anti-Galf antibody (L10) to detect the presence of Galf. Places where no medium was spotted are indicated with X; open circles indicate known Galf-minus mutants (1 = ΔugmA, 2 = 6.13#44/miaA, 3 = 15.4#17/miaB, 4 = 6.13#50/miaC); a mutant that did not grow in this experiment is indicated with X in an open circle; open squares indicate newly detected Galf-minus mutants (a = 15.4#5, b = 15.4#18, c = 15.4#30, d = 15.4#50, e = 15.4#57 and f = 6.47#41). 15.8, 6.13, 15.4 and 6.47 on top of each blot indicate the different parental backgrounds in which the mutants were made.