Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)

Table 3 Samples used for reference gene evaluation

Sample	Strain	Medium	Cultivation time and/or Condition
1	CBS 7435	YNBM	0 h
2	CBS 7435	YNBM, 1% Glu	8 h
3	CBS 7435	YNBM, 1% Glu	8 h
4	CBS 7435	YNBM, 1% Gly	8 h
5	CBS 7435	YNBM, 1% Gly	8 h
6	CBS 7435	YNBM, 1% MeOH	8 h
7	CBS 7435	YNBM, no C-source	8 h
8	CBS 7435	LB	8 h
9	CBS 7435	DMSZ, 1% Glu	8 h
10	CBS 7435	BMGY, 1% Glu	8 h
11	CBS 7435	YNBM, 1% Glu	pH = 3.0, 8 h
12	CBS 7435	YNBM, 1% Glu	pH = 7, 8 h
13	CBS 7435	YNBM, 1% Glu	37 °C, 8 h
14	CBS 7435	YNBM, 1% Glu	18 °C, 8 h
15	CBS 7435	YNBM, 1% Glu	2% EtOH, 8 h
16	CBS 7435	YNBM, 1% Glu	0.5 M NaCl, 8 h
17	CBS 7435	YNBM, 1% Glu	2% H₂O₂, 8 h
18	CBS 7435	YNBM, 1% Glu	10 µg/mL Zeocin, 8 h
19	CBS 7435	YNBM, 1% Glu	No agitation, 8 h
20	GS115	YNBM, 1% Glu	8 h
21	GS115	YNBM, 1% Gly	8 h
22	GS115	YNBM, 1% MeOH	8 h
23	GS115	LB	8 h
24	GS115	DMSZ, 1% Glu	8 h
25	GS115	BMGY, 1% Glu	8 h
26	BSYBG11	BSM, 1% MeOH	1 h
27	BSYBG11	BSM, 2% Gly	1 h
28	BSYBG11	BSM, 1% MeOH	8 h
29	BSYBG11	BSM, 2% Gly	8 h
30	BSYBG11	BSM, starvation	8 h after Gly depletion
31	BSYBG11	BSM	End Batch
32	BSYBG11	BSM	End Batch + MeOH pulse
33	BSYBG11	BSM	Fed-Batch, Methanol feed
34	BSYBG11	BSM	Start Fed Batch (Gly)
35	BSYBG11	BSM	End Fed Batch (Gly)

ISSN: 2054-3085