Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)

Table 1 RNA-Seq datasets used in this study

Sample ID	Accession	Platform	Run details	Culturing condition, sampling timepoint	Sample set	References
GSM4225310: B_10	SRX7415644	Illumina NovaSeq 6000	13.8G bases, 4.1 Gb	Glucose / Bench-scale batch growth fermentation at 10 h	Glucose	[33]
GSM4225313: M_12	SRX7415647	Illumina NovaSeq 6000	10.6G bases, 3.2 Gb	Methanol induction / Methanol growth 12 h	Methanol	[33]
GSM4225314: M_24	SRX7415648	Illumina NovaSeq 6000	11.5G bases, 3.5 Gb	Methanol induction / Methanol growth 24 h	Methanol	[33]
GSM4225311: G_3	SRX7415645	Illumina NovaSeq 6000	11.4G bases, 3.3 Gb	Glycerol growth at 3 h	Glycerol	[33]
GSM4025512: GS115.0G_A	SRX6691833	NextSeq 500	236.7 M bases, 96.1 Mb	24 h glycerol	Glycerol	[34]
GSM4025513: GS115.0G_B	SRX6691834	NextSeq 500	233.7 M bases, 95 Mb	24 h glycerol	Glycerol	[34]
GSM4025515: GS115.24G_B	SRX6691836	NextSeq 500	270.2 M bases, 109.4 Mb	48 h glycerol	Glycerol	[34]
GSM4225312: GM_2	SRX7415646	Illumina NovaSeq 6000	14.2G bases, 4.2 Gb	Glycerol and methanol mixture feed culture at 2 h	Glycerol and Methanol	[33]
GSM4025517: GS115.24M_A	SRX6691838	NextSeq 500	206.1 M bases, 83.4 Mb	24 h glycerol + 24 h methanol	Glycerol and methanol	[34]
GSM4025518: GS115.24M_B	SRX6691839	NextSeq 500	284.4 M bases, 114.6 Mb	24 h glycerol + 24 h methanol	Glycerol and methanol	[34]
GSM5155619: GS115	SRX10307452	HiSeq X Ten	4.7G bases, 1.8 Gb	YNBE (Yeast Nitrogen Base Ethanol) media for 14 h	YNBE	[35]

ISSN: 2054-3085