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Fig. 3 | Fungal Biology and Biotechnology

Fig. 3

From: A genetic tool to express long fungal biosynthetic genes

Fig. 3

Laetiporic acid production in the A. niger ∆fwnA mutants tLK04, tLK05 and tLK07. A Cultures photographs, representative domain structure of LpaA mutant proteins and HPLC profiles of metabolite extracts. Photographs depict 3-days cultures at 30 °C, 150 rpm. HPLC chromatograms of extracts of mycelia from tLK04, tLK05 and tLK07 were monitored at a wavelength of λ = 450 nm. Note, that tLK04 expressing the native lpaA gene produces laetiporic acids A1–D2, whereas no metabolites are detectable in tLK05 (expressing the mutant lpaAD1415A gene) or in tLK07 (empty vector control). Authentic standards of laetiporic acids (LA-A1/2, LA-B1/2 and LA-C1/2) served as controls. An authentic standard for LA-D1/2 was not available. For MS and MS/MS spectra please refer to Additional file 10: Fig. S6. The pearls on a string represent the domain structure of the PKS LpaA and include: KS, β-ketoacyl synthase; AT, acyl transferase; DH, dehydratase; MT, C-methyltransferase; ER0, (inactive) enoyl reductase; KR, keto reductase; ACP, acyl carrier protein; TE, thioesterase. B Chemical structure of laetiporic acids A1–D2. Note the common 1-methyl-2-oxo-propylidene group, which requires the action of the intrinsic C-MT domain

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