Fig. 2

Determination of the full-length integration of the PterA:lpaA:TtrpC construct into the fwnA genomic locus of the A. niger recipient strain tLK01. A Schematic representation of the integration event of the five lpaA fragments into the genomic fwnA locus of A. niger tLK01. To mutate the SAM binding site in the C-methyltransferase domain of LpaA, the triplet GAC (pos. 4245–4247) encoding Asp¹⁴¹⁵ (probably binding the ribose moiety of SAM) was site-mutated into the triplet GCC encoding Ala¹⁴¹⁵. B An agarose gel of a PCR targeting the PterA promoter and the TtrpC terminator has been performed (oligonucleotides oMG370/oMG116). The expected amplicon size is indicated. Full length integration was evident for seven tLK04 (producing native LpaA) and three tLK05 transformants (producing LpaA^D1415A). The genomic DNA of tLK07 (transformed with an empty vector) and the lpaA-encoding plasmid pPS03 [41] served as negative and positive controls, respectively