Table 2 The 30 most abundant proteins identified in EVs from A. pullulans

A0A4S9AIT8

5-Methyltetrahydropteroyltriglutamate-homocysteine methyltransferase

5-Methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity; zinc ion binding

methionine biosynthetic process

N/D

A0A074X7J3

NADP-specific glutamate dehydrogenase 1-related

Oxidoreductase activity

glutamate metabolic process; alpha-amino acid biosynthetic process

Cytosol

A0A4S8TEK2

Enolase

Hydro-lyase activity

glycolytic process

catalytic complex; cytosol

A0A074XWL5

Putative mitochondrial Hsp70 chaperone

ATP hydrolysis activity; ATP binding; heat shock protein binding; unfolded protein binding; misfolded protein binding

chaperone cofactor-dependent protein refolding; cellular response to unfolded protein

membrane; mitochondrion

A0A4T0BKW4

Glucose-6-phosphate isomerase

Small molecule binding; carbohydrate binding; isomerase activity

hexose biosynthetic process; glucose 6-phosphate metabolic process; glycolytic process; glucose metabolic process

cytosol

A0A074XKR6

Glyceraldehyde 3-phosphate dehydrogenase

Oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; NADP binding; F: NAD binding

glucose metabolic process

N/D

A0A4S8WPV8

Phosphoglycerate kinase

ATP binding; kinase activity

hexose biosynthetic process; glycolytic process; glucose metabolic process

cytosol

A0A4S8TCQ2

Transketolase

Transferase activity

generation of precursor metabolites and energy; glucose 6-phosphate metabolic process; NADP metabolic process

cytosol

A0A4S9SUA4

D-3-phosphoglycerate dehydrogenase 1-related

Oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; NAD binding

N/D

N/D

A0A4S9IG62

Glucokinase-1-related

Carbohydrate kinase activity; phosphotransferase activity, alcohol group as acceptor

Glucose 6-phosphate metabolic process; cellular glucose homeostasis; glycolytic process

membrane; mitochondrion; cytosol

A0A4S8V4V2

Inorganic diphosphatase

Inorganic diphosphatase activity; magnesium ion binding

phosphate-containing compound metabolic process

cytoplasm

A0A4T0E7S4

Protein MGARP

N/D

N/D

N/D

A0A4S9T4Y7

60 kDA heat shock protein

N/D

Protein folding

N/D

A0A4V4KL85

ATP synthase subunit beta

N/D

mitochondrial transmembrane transport; oxidative phosphorylation; mitochondrial transport; ATP synthesis coupled proton transport

Mitochondrial proton-transporting ATP synthase complex

A0A4S9MT33

ATP-citrate synthase

Acyltransferase activity

acyl-CoA metabolic process; amide biosynthetic process; purine ribonucleotide biosynthetic process; fatty acid biosynthetic process

Cytosol

A0A4S9LDW5

Inosine-5-monophosphate dehydrogenase related

Oxidoreductase activity, acting on the CH–OH group of donors, NAD or NADP as acceptor

purine ribonucleotide biosynthetic process; purine ribonucleoside triphosphate biosynthetic process

N/D

A0A1A7MQA9

60S ribosomal protein L4

RNA binding; structural constituent of ribosome

N/D

Cytosolic large ribosomal subunit

A0A1A7MK09

Pyruvate kinase

Intramolecular transferase activity; hydro-lyase activity; oxidoreductase activity; phosphotransferase activity, alcohol group as acceptor; kinase activity

glycolytic process

Cytoplasm

A0A074XJB5

Heat shock protein 83

Unfolded protein binding

cellular response to heat; protein folding; protein stabilization

Perinuclear region of cytoplasm; cytosol; plasma membrane

A0A074YII9

Hsc70Cb, isoform G-related

ATP binding; ATP-dependent protein folding chaperone

N/D

N/D

A0A1A7MKW1

Serine hydroxymethyltransferase

Carboxylic acid binding; zinc ion binding; heterocyclic compound binding; organic cyclic compound binding; small molecule binding; transferase activity, transferring one-carbon groups

Carboxylic acid catabolic process; cellular amide metabolic process; alpha-amino acid biosynthetic process; organonitrogen compound catabolic process; folic acid-containing compound metabolic process

Cytoplasm

A0A4S9ER00

ATP-citrate synthase

Acyltransferase activity

acyl-CoA metabolic process; amide biosynthetic process; purine ribonucleotide biosynthetic process; fatty acid biosynthetic process

Cytosol

A0A4S8WUD5

ATP synthase subunit alpha

ATP binding; proton transmembrane transporter activity; cation channel activity; ligase activity

ATP synthesis coupled proton transport

N/D

A0A074ZIJ5

Negative regulator of sporulation Mds3-related

N/D

Cellular homeostasis; regulation of cellular process

Membrane; mitochondrion; cytosol

A0A4S9ITR5

Ribosome-associated molecular chaperone Ssb1-related

ATP hydrolysis activity; ATP binding; heat shock protein binding; unfolded protein binding; misfolded protein binding

Vesicle-mediated transport; chaperone cofactor-dependent protein refolding; cellular response to unfolded protein

Nucleus; cytosol; plasma membrane

A0A4S9IQS0

Adenine phosphoribosyltransferase

Cation binding; anion binding; pentosyltransferase activity; purine ribonucleotide binding

Purine ribonucleotide biosynthetic process; nucleobase metabolic process; AMP metabolic process; pigment biosynthetic process

Cytoplasm

A0A1A7MJ01

Eukaryotic translation elongation factor 2

Translation elongation factor activity; GTPase activity; ribosome binding

Translational elongation

Cytosol; ribonucleoprotein complex

A0A074XC67

Polyketide synthase 1

Acyltransferase activity, transferring groups other than amino-acyl groups

Secondary metabolite biosynthetic process; fatty acid biosynthetic process

N/D

A0A4V4LMM1

Aconitate hydratase

Hydro-lyase activity; iron-sulfur cluster binding

Tricarboxylic acid cycle

Cytosol

A0A1A7MP02

Phosphohexomutase family member

Intramolecular transferase activity

Carbohydrate metabolic process

Cytosol