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Table 4 Components of the digestive EV treatments used to measure the effects of treatment reagents on fungal growth

From: Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Controls

C5 (µl)

C6 (µl)

C7 (µl)

C8 (µl)

C9 (µl)

PBS

55.8

50

57

58.8

54.2

RNase

1.2

1.2

 

1.2

 

PK

3

3

3

  

Triton X

 

5.8

  

5.8

Total

60

60

60

60

60

  1. Investigated concentrations: Proteinase K (20 ng/µl) (Thermo Fisher Scientific); RNase A (20 ng/µl) (Thermo Fisher Scientific); 10% Triton X-100 (Sigma)