Fig. 6

42 h post-inoculation (hpi) EV–F. graminearum co-cultures were harvested and technical triplicates were combined before RNA isolation. Transcriptional analyses were performed and FgCYP51A and FgCYP51C expression was calculated with the ΔΔCt method using the elongation factor 1 α as the reference gene. ΔΔCt values were calculated by referring to ΔCt values of samples which were derived from F. graminearum cultures where EVs were equally treated after isolation but derived from control-sprayed (non-RNA) leaves