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Fig. 7 | Fungal Biology and Biotechnology

Fig. 7

From: Characterisation of ascocorynin biosynthesis in the purple jellydisc fungus Ascocoryne sarcoides

Fig. 7

Phenotypic and metabolite analysis of A. oryzae OP12 strains expressing the polyporic acid synthetase gene acyN with or without co-expression of the MO6277 gene. a Strains were either grown for 60 h on potato dextrose agar plates (left) or for 24 h in liquid minimal medium with 2% starch as carbon source (right). An A. oryzae OP12 strain without expression construct served as control. When only the acyN gene is expressed, growth on plates and mycelium formation in liquid medium is strongly retarded and polyporic acid strongly attaches to the mycelium. Co-expression of the monooxygenase partially restores growth, and the produced pigments show increased solubility in the medium. b HPLC analysis of extracts from strains shown in (a). Polyporic acid produced in the A. oryzae strain expressing only the acyNOPT gene is nearly quantitatively converted into ascocorynin. None of the strains produces atromentin (added as control)

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