Fig. 6
From: Characterisation of ascocorynin biosynthesis in the purple jellydisc fungus Ascocoryne sarcoides
Semiquantitative RT-PCR analysis of all monooxygenase-encoding genes from A. sarcoides. The same cDNA from A. sarcoides strain (CBS 247.80) and strain SC1 was used as in the analysis of the ascocorynin biosynthesis gene cluster expression analysis. Potato dextrose broth (PDB) acts as ascocorynin-inducing and Aspergillus complete medium (ACM) as non-inducing condition. The beta-tubulin gene served for normalisation of cDNA concentrations and genomic DNA (gDNA) was used as PCR control. Only the monooxygenase MO6277 (boxed) shows the same expression pattern as the acyN gene