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Table 3 Phenotypes on PSA of isolates from putative PKS12 gene variants

From: CRISPR/Cas9 mediated editing of the Quorn fungus Fusarium venenatum A3/5 by transient expression of Cas9 and sgRNAs targeting endogenous marker gene PKS12

  

Phenotype

 

Putative variant

Number of isolates

Red

Albino

% albino phenotypes

pFC332 control

4

4

–

0

5S-1

7

–

7

100

5S-2

7

–

7

100

5S-4

10

–

10

100

5S-5

9

–

9

100

5S-6

10

–

10

100

5S-7

10

–

10

100

5S-10

10

–

10

100

PolII-1

12

12

–

0

PolII-2

9

9

–

0

PolII-3

9

9

–

0

PolII-4

14

14

–

0

PolII-5

10

7

3

30

PolII-6

10

6

4

40

PolII-7

11

7

4

36

PolII-8

10

10

–

0

PolII-9

10

9

–

0

PolII-12

10

9

–

0

PolII-13

10

10

–

0

  1. Putative PKS12 gene variants were generated using sgRNAs transcribed from the PolIII promoter PFv5SrRNA (5S) or the PolII promoter PgdpA (PolII). Control vector pFC332 is without sgRNA constructs. Isogenic isolates were cultured on potato sucrose agar (PSA), which induces red pigmentation in strains with functional polyketide synthase encoded by the PKS12 gene. PKS12 frameshift variants have an albino phenotype.
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Fungal Biology and Biotechnology

ISSN: 2054-3085

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